Several techniques are available at Bio-Synthesis to evaluate the quantity, quality,
and function of the antibodies produced. BSI's Protein Analytical Methods can provide
you with important and detailed information about polyclonal and monoclonal antibodies
and help you throughout the development phase.
Bio-Synthesis can provide you with detailed characterization of your antibody including:
- Antibody titration and quantization by ELISA
- Western Blot Assay
- Luminex Assay Antibody Assay
- HPLC, SDS, PAGE IEF Analysis
- Intact mass determination by mass spectrometry of heavy and
light chain
- N- and C-terminal sequencing of heavy and light chain by Edman
degradation and MALDI MS
To obtain further information, please contact us.
Luminex Antibody Assay Services
The multiplex Luminex® assay format differs from conventional ELISA in one significant
way - the multiplex capture antibody is attached to a polystyrene bead whereas the
ELISA capture antibody is attached to the microplate well.
Luminex assays are based on xMAP technology (multi-analyte profiling beads) enabling
the detection and quantitation of multiple RNA or protein targets simultaneously.
The xMAP system combines a flow cytometer, fluorescent-dyed microspheres (beads),
lasers and digital signal processing to effectively allow multiplexing of up to
100 unique assays within a single sample.
Bio-Synthesis can guide you through all the steps starting from your needs finishing
with your multiplex immunoassay.
- Project design
- Ab production and QC
- Coupling to microsphere
- Monoplex setup
- Multiplex setup
- Testing on biological material
See details on Luminex Assay Development and Services.
ELISA Development and Testing
ELISAs (Enzyme Linked Immunosorbent Assays) are used to measure the specific binding
between an antigen and antibody using a secondary enzyme labeled antibody and substrate
system. This is a rapid and sensitive method for evaluating large numbers of monoclonal
or polyclonal antibodies at low cost. The scientists at BSI have years of experience
developing ELISAs for our clients and for our own in-house antibodies and we are
pleased to share that experience with you. Once an ELISA has been developed for
you, we will transfer the ELISA protocol to you and, if you wish, apply that ELISA
to testing of your antibody samples.
Western Blot Assay and Electrophoresis
This technique permits identification of proteins by their molecular weight and
their immunoreactivity or the control of the immunoreactivity of serum on the denaturated
protein of interest. The sample is prepared and loaded into a pre-cast 10% to 12%
Tris-HCl denaturing gel. The standards are included with molecular weights ranging
from 6.5kD to 200kD. Two bands are observed after staining in Coomassie Blue rep-
resenting the heavy chain (55kD) and the light chain (25kD) of the antibody. Each
client is provided with a photo of the gel as an indicator of purity.
Type of sample:
- Purified protein: 15-20 µg
- Cellular lysate containing the protein of interest: 500 µg
Isotype Determination
The isotype of murine antibodies (either in supernatants or ascites) can be determined
using murine isotyping kits or strips.
For additional antibody characterization services, visit our
proteomic analytical services.