Antibody Liposome Bioconjugation
Antibody conjugated liposomes have recently attracted a great deal of interest, principally because of their potential use as targeted drug delivery systems and in diagnostic applications. Conjugation of an antibody to a liposome can be time consuming and result in a low yield due to the instability of the linkers on both the liposome and the antibody to be conjugated. Bio-Synthesis can help you save time and hassle by providing our clients with high quality antibody-oligonucleotide conjugates using the most advanced bioconjugation chemistry. Our scientific staff has extensive experience in the field of developing unique bioconjugation strategies for protein, antibody, peptide, and oligonucleotide. If you are in need of developing any drug bioconjugates, diagnostic bioconjugates, or research bioconjugates, Bio-Synthesis is here to help you.
Chemistries
Antibody is thiolated with 2-iminothiolane and then reacted with a maleimide-activated Liposome.
Note:
In this case sulfhydryl functional groups will be introduced to an antibody through Lys residues using a reliable and reproducible procedure. However, if some of the modified or conjugated Lys residues are located on the antigen binding sites, this method may produce partially active or inactive antibody-enzyme conjugates that may not bind to the antigen. In such a case an alternative conjugation method may be used.
Quality Control Criteria
The custom prepared product is quality controlled by Bio-Synthesis according to our in-house standards. These are tailored for each individual assignment. The custom conjugate is approved only when both sets of quality control criteria have been met.
Custom Formulation
Our conjugation is based on a mixed short length polymer cross linked covalent bonded technique. We require ligands contain certain function group for conjugation, please contact our technical support for details.
Prices
Due to the nature of each project, a written quotation will be made stating cost and time required after receipt of the antibody sample.
Standard price covers labor and materials including the cost of maleimide -activated oligo preparation, antibody thiolation, conjugation procedure, conjugate purification and analysis.
Delivery
Conjugations take 2-3 weeks. All shipments are sent Fed Ex Standard Overnight delivery.
*No shipments on Fridays
Sample Submission Guideline
As a rule, Bio-Synthesis will only accept pure substances as starting material, either lyophilized or dissolved in a medium of known chemical composition. Interfering substances must not be present. Non-commercial antibodies supplied by the customer should
be affinity purified. Please provide gel eletrophoresis data along with your antibody. Commercially available antibodies can be
supplied by customers or ordered through Bio-Synthesis. The cost of acquiring antibodies will be added to the invoice plus an administration charge (15% of total cost). We can assist in antibody modification or fragmentation prior to any immobilization reaction.