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Home » Antibody and Immunochemistry » Polyclonal Antibody Services » Select By Host Animals » Chicken Polyclonal Antibody

Custom Chicken Polyclonal Antibody

Bio-Synthesis (BSI) provides chicken IgY polyclonal antibody production in its USDA-licensed facility using Sex-Sal or Leghorn Hen. Chickens produce an immunoglobulin (IgY), which can be isolated from blood or egg yolk. Compared to the more familiar mammalian IgG antibodies, chicken IgY antibodies not only have higher affinity and avidity but also lower cross-reactivity. The following are benefits of choosing chicken IgY polyclonal antibodies:

  • High yields: Each egg yolk contains approximately 100 mg of antibody (IgY) before purification
  • Large quantities: Each chicken will average one egg every 2-3 days, no need to bleed the animal.
  • Non-mammalian system: Chickens are not mammals and therefore are more apt to making high affinity and avidity antibodies to mammalian antigens. For highly conserved mammalian proteins, raising antibodies in an avian (chicken) system is often a preferred alternative.
These advantages are rendering chicken IgY antibodies more popular in laboratory research and point-of-care (POC) diagnostics. See details to learn more about IgY

We can produce chicken IgY antibody with a wide variety of antigens, from bacteria, viruses, fungi, fusion proteins, synthetic peptides, and plasmid DNA, as well as a variety of other proteins. Typical antigen requirement for is 2.0 mg conjugated peptide/animal, and 1.0 mg protein antigen/animal. However, amounts may vary due to antigen availability and additional antigen is required for affinity purification. Additional animals are available for all service packages.

One production schedule is available for our chicken IgY polyclonal antibody services. We can produce polyclonal IgY antibodies using our standard protocol or by a customer-designed protocol. We also supply polyclonal production from other species, including rabbit, guinea pigs, goat, sheep, rat, and mouse.

BSI supplies a Polyclonal Antibody Information Sheet with each inquiry to assist in meeting our customer specifications.

Custom Chicken Antibody Service Pacakges

Don't see what you need? Just ask - in most cases we can accommodate your request. If you require help in choosing the services for your polyclonal antibody production, feel free to consult our experts.

All of our antibody packages can be prolonged with an additional immunization every 28 days which includes two additional bleeds after the immunization. Production extension is an additional charge of animal maintenance fee.

Request a quote or contact us for your Chicken Polyclonal Antibody packages.
Service Descriptions Antigen Packages   Purification
 
Peptide
Protein
Affinity Conjugation IgY A
SP-PE-CH120Y Super Class Plus IgY Purified (Chicken) Antibody: peptide synthesis >90%, conjugation, serum production, and IgY purification √     √ √  
SP-PE-CH120IY Super Class Combo IgY Purified (Chicken) Antibody: peptide synthesis >90%, conjugation, serum production, peptide-specific affinity IgY from yolk √   √ √ √  
US-PR-CH-120Y User-supplied Protein Plus IgY Purified (Chicken) Antibody: IgY purification   √     √  
US-PR-CH120IY User-supplied Protein Combo IgY Purified (Chicken) Antibody: antigen-specific affinity yolk IgY purification from Yolk   √ √   √  
Three production schedules are available for each Chicken Polyclonal Antibody package along with its descriptions.

Available Production Time Frame:
  • 70-90 days
Custom Peptide Polyclonal Antibody Services
Super Class Peptide Plus IgY Purified Antibody Package (Chicken)
Package: SP-PE-CH120Y (70 Days Immunization Protocol) Time Frame 70-90 days and delivery
Peptide Synthesis > 90% (<20 a.a. 15 mg) ~5 ml control serum and 1 control egg per chicken
KLH/BSA conjugation via Cysteine added on C- or N-term of peptide up to 90 ml crude antiserum and remaining egg yolks
2 chickens, 2 control serum (1/animal), 2 control eggs (1/animal), 2 initial inoculations (1/animal), 8 boosters (4/animal), 6 production serum (3/animal), 22 collection eggs (11/animal) IgY purified antibodies from 100 ml of egg yolks
IgY purfication from yolk 5 mg of unconjugated peptide
ELISA titer assay on collection serum and IgY purified samples MS, HPLC of peptide, ELISA assay report
SDS PAGE analysis Electronic image of SDS analysis
  See immunization and delivery schedule.
 
Super Class Peptide Combo IgY Purified Antibody Package (Chicken)
Package: SP-PE-CH120IY (70 Days Immunization Protocol) Time Frame 70-90 days and delivery
Peptide Synthesis > 90% (<20 a.a. 20 mg) ~5 ml control serum and 1 control egg per chicken
KLH/BSA conjugation via Cysteine added on C- or N-term of peptide up to 90 ml crude antiserum and remaining egg yolks
2 chickens, 2 control serum (1/animal), 2 control eggs (1/animal), 2 initial inoculations (1/animal), 8 boosters (4/animal), 6 production serum (3/animal), 22 collection eggs (11/animal) Peptide Affinity purified IgY from 100 ml of yolk (~ 6 eggs)
Preparation for two purification columns 5 mg of unconjugated peptide
IgY and peptide-specific affinity purification from 100 ml of yolk (~ 6 eggs) MS, HPLC of peptide, ELISA reports
ELISA titer assay on collection serum and peptide affinity purified samples Electronic image of SDS analysis
SDS PAGE analysis See immunization and delivery schedule.
 
User Supplied Immunogen - Peptide/Recombinant and Fusion Protein
User Supplied Protein Plus IgY Purified Antibody Package (Chicken)
Package: US-PR-CH120Y (70 Days Immunization Protocol) Time Frame 70-90 days and delivery
Conjugation to carrier (optional, additional fee) ~5 ml control serum and 1 control egg per chicken
2 chickens, 2 control serum (1/animal), 2 control eggs (1/animal), 2 initial inoculations (1/animal), 8 boosters (4/animal), 6 production serum (3/animal), 22 collection eggs (11/animal) up to 90 ml crude antiserum and remaining egg yolks
IgY purfication from yolk IgY purified antibodies from 100 ml of egg yolks (~ 6 eggs)
ELISA titer assay of collection serum and IgY purified samples ELISA report and electronic image of SDS analysis
SDS-PAGE analysis See immunization and delivery schedule.
Antigen requirement: 2-4 mgs of protein. The concentration should be 1 mg/ml is a good target range, minimum >0.5 mg/ml in PBS buffered solution pH 7.4.
See details on antigen preparation
 
User-Supplied Protein Combo IgY Purified Antibody Package (Chicken)
Package: US-PR-CH120IY (70 Days Immunization Protocol) Time Frame 70-90 days and delivery
Conjugation to carrier (optional, additional fee) ~ 5 ml control serum and 1 control eggs per chicken
2 chickens, 2 control serum (1/animal), 2 control eggs (1/animal), 2 initial inoculations (1/animal), 8 boosters (4/animal), 6 production serum (3/animal), 22 collection eggs (11/animal) Protein-Specific affinity purified IgY from 100 ml of yolk (~ 6 eggs)
Preparation for two purification columns up to 90 ml crude antiserum and remaining egg yolks
Protein specific affinity column preparation Proteic bounded affinity column
Protein specific Affinity purification from 100 ml of yolk IgY and antigen-specific affinity purificiation from 100 ml of yolk (~ 6 eggs)
ELISA titer assay of collection serum and peptide affinity purified samples ELISA report
SDS-PAGE analysis Electronic image of SDS analysis
  See immunization and delivery schedule.
Antigen requirement: 10 mgs of protein (2-4 mgs for immunization, 5 mgs for purification) The concentration should be 1 mg/ml is a good target range, minimum >0.5 mg/ml in PBS buffered solution pH 7.4.
See details on antigen preparation
 
We offer two standard custom antibody protocols (combinations of hosts and protocol time frame) From peptide design, synthesis, and conjugation, to antibody generation and purification, Bio-Synthesis offers a complete package to meet your protein detection needs. User-design production protocol also available and can also be modified from existing package.
Serum Production Specification:
Immunization schedule listed below are approximate (± 2 days).

  • Two White Leghorn chickens will be utilized for each MAP-peptide, conjugated- peptide immunogen, or protein immunogen packages
  • Total material required for injection for the program below is ~1.0 mg protein/animal, ~2.0 mg peptide/animal conjugate. If we are conjugating your protein we assume a 20% loss of protein during conjugation. Total amount of serum expected from a chicken on the program below is approximately ~45 mL/animal, 12 eggs.
  • Immunogen is emulsified in Freund's Complete Adjuvant (FCA) for initial injections. Freund's Incomplete Adjuvant (FIA) is used for all subsequent injections (boosts).
  • Continuation of project past our standard immunization schedule is offered on a month to month basis. The standard extension protocol includes one booster, two serum collection, and maintenance for one month. Terminal bleeds may be obtained at any time yielding approximately ~50 ml/animal of additional serum taken upon customer request.
 
Program Development of Chicken Polyclonal Antibody: 70-90 Days
Procedure Protocol Days* Description
Phase I - Antigen design, synthesis and preparation
Antigen Preparation ~ 10 - 15 days Peptide synthesis and purification (if required) and antigen conjugation
Phase II - Immunization Schedule
Control serum collection



Initial Inoculation 		Day 0 2 White Leghorn Chickens -2 pre-immune eggs and collection of control serum (~5 ml serum/chicken)

Initial Inoculation with 500 µg peptide conjugates or 250 µg protein per host with FCA in SQ 4 sites
1st Booster Day 14 Boost with 500 µg peptide conjugates or 125 µg protein per host with FIA in SQ 4 sites
2nd Booster Day 28 Boost with 250 µg peptide conjugates or 125 µg protein per host with FIA in SQ 4 sites
Egg Collection for 2 days

1st Collection serum

3rd Booster 		Day 41 - 42 ~2 egg yolks/chicken

Week 6 Collection serum ~15 mls/chicken

Boost with 250 µg peptide conjugates or 125 µg protein per host with FIA in SQ 4 sites
Egg Collection for 3 days

2nd Collection serum

4th Booster 		Day 54 - 56 ~3 egg yolks/chicken

Week 8 Collection serum ~15 mls/chicken

Boost with 250 µg peptide conjugates or 125 µg protein per host with FIA in SQ 4 sites
ELISA Titer Assay

1st Shipment

Project Completion Verification 		Day 60 ELISA assay of control, week 8 collection serum

Shipping of  control, 6 and 8 week collection serum and egg yolks,  ELISA data

Verify disposition of chickens, extension or termination
Egg Collection for 6 days

3rd Collection serum 

2nd Shipment 		Day 65 - 70 ~6 egg yolks/chicken

Week 10 Collection serum ~15 mls/chicken

 Shipping of 10 week collection serum and egg yolks
Instruction Due Date Day 87 Termination of animals if instruction not received
 
Links and Resources

Forms:
Order/Quote Page

Additional Phospho-Specific Antibody Information:
Phospho-Specific Life-Cycle Illustration

Sci Apps:
Peptide Property Calculator
Peptide Design Library
Oligo Designer

Useful Links
Reference/Citing:
1.
Different prelamin A forms accumulate in human fibroblasts: a study in experimental models and progeria
2.
1,25-DIHYDROXYVITAMIN D3-INDUCED GENES IN OSTEOBLASTS: UNCOVERING NEW FUNCTIONS FOR MENINGIOMA 1 AND SEMAPHORIN 3B IN SKELETAL PHYSIOLOGY
3.
Fetal Death in Mice Lacking 5α-Reductase Type 1 Caused by Estrogen Excess
4.
A splicing variant of a death domain protein that is regulated by a mitogen-activated kinase is a substrate for c-Jun N-terminal kinase in the human central nervous system
Ordering Services
To place an order or request a quotation, please proceed to our custom Order/Quote page or Contact Us for any other questions.
Order can be placed with either a PO (Purchase Order) or credit card. You may place order online using our secure online ordering system. Our customer service representatives are available 24 hours a day, Monday through Friday, to assist you.
   
We accept POs and major credit cards ( ). Your credit card will be billed under "Bio-Synthesis, Inc." Please complete and return our Business Credit Application. For international orders, we must apply the full charge at the time of the order is placed. In the unlikely event that any given order cannot be filled, our guarantee will take the form of a full refund.
 
 
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