Aldehyde
Aldehydes are attractive electrophiles for bioconjugation, since they react with
nucleophiles such as amines to form a Schiff's base, with hydrazino groups to form
hydrazones, and with semicarbazides to form semi-carbazones. The Schiff’s base is
unstable and must be reduced with sodium borohydride to form a stable linkage but
hydrazones and semicarbazides are very stable linkages.
|
Aldehyde C2
|
Desalted Qty.
|
5'-Mod
|
Internal
|
3' -mod
|
|
250 nmol
|
15 OD
|
$85
|
NA
|
NA
|
|
1.0 µmol
|
35 OD
|
$85
|
NA
|
NA
|
|
Purification
Desalt or cartridge (RP1) purification is acceptable for most common phosphoramidite
modifiers. However, additional purification is strongly recommended for modifiers
that require NHS-ester chemistry to conjugate a dye through an amine linker such
as Molecular Probes dyes.
Minimum Guarantee Purified Yield
Due to the chemistry of many modifications, yield will be approximately 40 - 60
% less than a standard oligo.
Please see our yield chart for details.
Quality Controls
Every oligo synthesized
is strictly controlled for quality by using either MALDI-TOF
mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields
are determined using UV absorbance at OD260 In addition, we perform QC
methods tailored to specific modifications, such as OD ratio measurement where appropriate.
For additional information, please Contact us
The most trusted
name in Quality Custom Synthesis