Alexa Dye Series

These dyes are available as NHS Esters and are conjugated post synthesis to an amino-modified oligo and two step HPLC purifications are required. The spectral diversity of the Alexa Fluor dyes make them ideal tools for multicolor applications such as fluorescence in situ hybridization (FISH) and microarray experiments. Our wide selection of dyes spans the visible spectrum and beyond, providing enormous flexibility in choosing a label that is compatible with microarray scanners and fluorescence microscopes. The 

Alexa Fluor dyes

 have several properties that make them superior to other fluorescent dyes:

Oligonucleotides labeled with Alexa Fluor have many advantages:

•  High water solubility.The Alexa Fluor dyes are highly water soluble, making them ideal for hybridization experiments. Nucleic acids labeled with the Alexa Fluor dyes do not aggregate or precipitate, even in high-salt conditions.

•  pH independence. Fluorescence of the Alexa Fluor conjugates is not pH sensitive in the ranges used for hybridization solutions and microscopy mounting media.

•  Resistance to photobleaching. The enhanced photostability of Alexa Fluor dyes makes them ideal for applications requiring imaging, such as FISH and microarrays.

•  High signal correlation.

The Alexa Fluor 555/Alexa Fluor 647 dye pair provides better signal correlation and therefore higher resolution of diff erentially expressed genes than the commonly used Cy3/Cy5 dye pair

Similar alternatives include the DyLight Fluors and the Atto.

Contact your Account Representative for pricing on Alexa dye labeled oligonucleotides. For additional information, please Contact us.

Price/labeling, purification and synthesis is an additional charge