NEBcutter is an on-line tool for restriction mapping nucleotide sequences that also features identification of non-overlapping ORFs.
Alternative restriction enzyme analysis..
Convert DNA sequence data to it's Reverse Complement.
Translate DNA sequence in all 6 possible frames.
There are two sites you can use. The preferred one is located at www.justbio.com. You will need to register yourself with this site before you can use this tool and you must use Internet Explorer V5.5 (or better) or Netscape V6 (or better). This facility allows translation of DNA sequence in one, or more frames. The output is formatted as free text amino acid sequence as well as DNA sequence plus the aligned translation. This utility is a useful and fast way to format your sequence following an Open Reading Frame analysis.
Graphically identify ORFs within DNA sequence data.
A neural network based program to find possible transcription promoters. From the Berkley Drosophila Genome Project
Screens DNA sequences for interspersed repeats and low complexity DNA sequences. Multiple sequences may me submitted in FASTA format
Find inverted and tandem repeats in sequence data.
Java Script Oligo calculator (Netscape V2 or better).
Differences in codon usage among organisms can lead to a variety of problems concerning heterologous gene expression. The GCUA tools display the codon bias in a graphical manner.