ATTO Fluorophore Labeling
New set of excellent
fluorescent dyes from ATTO-Tec has added to our broad portfolio
of labels for oligonucleotides. These activated fluorescent dyes have become the
standard procedure for stable labelling of proteins, nucleic acids, ligands and
other biomolecules, which are widely used for applications as fluorescence microscopy,
flow cytometry, fluorescence in situ hybridisation (FISH), receptor binding assays,
or enzyme assays.
ATTO dyes are a series of fluorescent dyes which provide all the crucial properties
required for modern fluorescent technologies.
- Strong absorption (high extinction coefficient)
- High fluorescence quantum yield
- High photostability
- Good water solubility
- Low triplet formation
- Ideally suited for bioanalytical applications
This compound requires amino- or thiol- linker with a 6-Carbon spacer arm or by
substituting any Amino Modifier C6 dA, dT, dG, dC for internal labeling, a double
HPLC purification is required.
Contact your Account Representative for pricing on ATTO labeled oligonucleotides. For additional information, please
Contact us
Price/labeling, purification and synthesis is an additional charge
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Dye Groups
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Scales
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Label position
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5'
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Int
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3'
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ATTO390
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250 nmol
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$318
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Inquire
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$318
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1.0 µmo
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Inquire
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Inquire
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Inquire
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ATTO465
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250 nmol
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$246
|
Inquire
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$246
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1.0 µmo
|
Inquire
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Inquire
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Inquire
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ATTO465
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250 nmol
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$246
|
Inquire
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$246
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1.0 µmo
|
Inquire
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Inquire
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Inquire
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ATTO488
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250 nmol
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$395
|
Inquire
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$395
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1.0 µmo
|
Inquire
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Inquire
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Inquire
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ATTO495
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250 nmol
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$288
|
Inquire
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$288
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1.0 µmo
|
Inquire
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Inquire
|
Inquire
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ATTO520
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250 nmol
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$260
|
Inquire
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$260
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1.0 µmo
|
Inquire
|
Inquire
|
Inquire
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ATTO532
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250 nmol
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$390
|
Inquire
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$390
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1.0 µmo
|
Inquire
|
Inquire
|
Inquire
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ATTO540 Quencher
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250 nmol
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$450
|
Inquire
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$450
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1.0 µmo
|
Inquire
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Inquire
|
Inquire
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ATTO550
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250 nmol
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$390
|
Inquire
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$390
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1.0 µmo
|
Inquire
|
Inquire
|
Inquire
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ATTO565
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250 nmol
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$273
|
Inquire
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$273
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1.0 µmo
|
Inquire
|
Inquire
|
Inquire
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ATTO580 Quencher
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250 nmol
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$450
|
Inquire
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$450
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1.0 µmo
|
Inquire
|
Inquire
|
Inquire
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ATTO590
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250 nmol
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$280
|
Inquire
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$280
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1.0 µmo
|
Inquire
|
Inquire
|
Inquire
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ATTO594
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250 nmol
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$390
|
Inquire
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$390
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1.0 µmo
|
Inquire
|
Inquire
|
Inquire
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ATTO610
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250 nmol
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$215
|
Inquire
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$215
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1.0 µmo
|
Inquire
|
Inquire
|
Inquire
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ATTO612 Quencher
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250 nmol
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$450
|
Inquire
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$450
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1.0 µmo
|
Inquire
|
Inquire
|
Inquire
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ATTO620
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250 nmol
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$450
|
Inquire
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$450
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1.0 µmo
|
Inquire
|
Inquire
|
Inquire
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ATTO633
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250 nmol
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$450
|
Inquire
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$450
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1.0 µmo
|
Inquire
|
Inquire
|
Inquire
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ATTO637
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250 nmol
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$450
|
Inquire
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$450
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1.0 µmo
|
Inquire
|
Inquire
|
Inquire
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ATTO647N
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250 nmol
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$450
|
Inquire
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$450
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1.0 µmo
|
Inquire
|
Inquire
|
Inquire
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ATTO655
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250 nmol
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$450
|
Inquire
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$450
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1.0 µmo
|
Inquire
|
Inquire
|
Inquire
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ATTO680
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250 nmol
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$450
|
Inquire
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$450
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1.0 µmo
|
Inquire
|
Inquire
|
Inquire
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ATTO700
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250 nmol
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$450
|
Inquire
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$450
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1.0 µmo
|
Inquire
|
Inquire
|
Inquire
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ATTO720
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250 nmol
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$450
|
Inquire
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$450
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1.0 µmo
|
Inquire
|
Inquire
|
Inquire
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ATTO740
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250 nmol
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$450
|
Inquire
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$450
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1.0 µmo
|
Inquire
|
Inquire
|
Inquire
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Purification
Desalt or cartridge (RP1) purification is acceptable for most common phosphoramidite
modifiers. However, additional purification is strongly recommended for modifiers
that require NHS-ester chemistry to conjugate a dye through an amine linker such
as Molecular Probes dyes.
Minimum Guarantee Purified Yield
Due to the chemistry of many modifications, yield will be approximately 40 - 60
% less than a standard oligo.
Please see our yield chart for details.
Quality Controls
Every
oligo synthesized is strictly controlled for quality by using either MALDI-TOF
mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final
yields are determined using UV absorbance at OD
260. In addition,
we perform QC methods tailored to specific modifications, such as OD ratio measurement
where appropriate.
For additional information, please
Contact us.
The most trusted
name in Quality Custom Synthesis