Bodipy Dye Oligo Labeling

Custom DNA Synthesis--"FASTEST services in the industry"

BODIPY® Fluorophores

Bodipy® Dyes have a narrow spectral characteristics and are highly fluorescent that are often superior to those of fluorescein, tetramethylrhodamine, Texas Red and longer-wavelength dyes. These dyes are unusual in that they are:

• Higher quantum yields
• Narrower emission bandwidths
• Greater photostability
• Insensitive to pH changes
• Useful for DNA sequencing because the dye exhibits minimal effect on the mobility of the fragment during electrophoresis

Bodipy labels are attached at the 5'- , Internal, or 3'- end of an oligonucleotide. In a post-synthetic step, the dye is coupled to an amino-modified oligo.

This compound requires amino- or thiol- linker with a 6-Carbon spacer arm or by substituting any Amino Modifier C6 dA, dT, dG, dC for internal labeling, a double HPLC purification is required.

Contact your Account Representative for pricing on BODIPY labeled oligonucleotides. For additional information, please contact us.

Price/labeling, purification and synthesis is an additional charge

Dyes	Scales	Scales
		5'	Int	3'
BODIPY 493/503TM	250 nmol	$325	Inquire	$325
	1.0 umol	$375	Inquire	$375
BODIPY 558/568TM	250 nmol	$325	Inquire	$325
	1.0 umol	$375	Inquire	$375
BODIPY 564/570TM	250 nmol	$325	Inquire	$325
	1.0 umol	$375	Inquire	$375
BODIPY 576/589TM	250 nmol	$325	Inquire	$325
	1.0 umol	$375	Inquire	$375
BODIPY 581/591TM	250 nmol	$325	Inquire	$325
	1.0 umol	$375	Inquire	$375
BODIPY TMR-XTM	250 nmol	$325	Inquire	$325
	1.0 umol	$375	Inquire	$375
BODIPY TR-XTM	250 nmol	$350	Inquire	$350
	1.0 umol	$395	Inquire	$395
BODIPY 530/550TM	250 nmol	$325	Inquire	$325
	1.0 umol	$375	Inquire	$375
BODIPY-FL BR2TM	250 nmol	$325	Inquire	$325
	1.0 umol	$375	Inquire	$375
BODIPY-FL-XTM	250 nmol	$325	Inquire	$325
	1.0 umol	$375	Inquire	$375

Purification

A wide variety of modifications can be incorporated into an oligonucleotide at the time of synthesis, using aspecialized phosphoramidite chemistries for labeling. However, certain modifications require the use of post-synthetic conjugation strategies in which a primary amine modified oligonucleotide is covalently attached to a dye via NHS ester chemistry. Other cross-linking chemistries are also available depending on particular project specifications. Single HPLC purification is recommended when using phosphoramidite oligo chemistry, otherwise dual HPLC is required for post-synthetic labeling.

Minimum Guarantee Purified Yield

Due to the chemistry of many modifications, yield will be approximately 40 - 60 % less than a standard oligo. Please see our yield chart for details.

Quality Controls

Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis.  Final yields are determined using UV absorbance at OD₂₆₀.  In addition, we perform QC methods tailored to specific modifications, such as OD ratio measurement where appropriate. 

For additional information, please contact us.