Photocleavable Biotin Modifier
PC Biotin can be use for capture by streptavidin in a mode similar to our popular
5' Biotin oligonucleotide. DNA researchers have long sought effective ways to capture
oligonucleotides or PCR products from a crude mixture and then to release them in
a pure, biologically active form. Now such a technique developed by AmberGen, Inc.
is available using PC Biotin and related photocleavable (PC) modifiers. After capturing
biotin labeled DNA with streptavidin beads or attaching modified DNA to a surface,
the DNA can be released into solution by simply illuminating with a hand-held UV
light source.1 Moreover, once freed from its tether the DNA is biologically active.
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PC-LC Biotin
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Desalted Qty.
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5'-Mod
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Internal
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3' -mod
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250 nmol
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15 OD
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$150
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NA
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NA
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1.0 µmol
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35 OD
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$280
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NA
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NA
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Purification
Desalt or cartridge (RP1) purification is acceptable for most common phosphoramidite
modifiers. However, additional purification is strongly recommended for modifiers
that require NHS-ester chemistry to conjugate a dye through an amine linker such
as Molecular Probes dyes.
Minimum Guarantee Purified Yield
Due to the chemistry of many modifications, yield will be approximately 40 - 60
% less than a standard oligo.
Please see our yield chart for details.
Quality Controls
Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF
mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields
are determined using UV absorbance at OD260 In addition, we perform QC
methods tailored to specific modifications, such as OD ratio measurement where appropriate.
For additional information, please Contact us.
The most trusted
name in Quality Custom Synthesis