Dyomic Dye Oligo Labeling

Custom DNA Synthesis--"FASTEST services in the industry"

Dyomics ® Fluorophores Labeling

We offer DY-labels oligonucleotide from Dyomics with more than 50 fluorophores covering the whole fluorescence spectrum. These coupled oligonucleotides are used in cDNA detection on microarray, fluorescence assays like FISH, Real time PCR or FRET assays and they may be an attractive alternative for traditional fluorescence dyes. Dy 647 is a bright orange dye with similar properties to CY^®5 and Alex Fluore^® 647, DY547 is also bright organic dye with similar properties to CY^®3.

Other dyes (called Mega Stokes-Dyes) exhibit a large shift of fluorescence relative to the absorption (Stokes shift).

DY 480XL, 461XL and DY520XL, these dyes are compatible with the use of Taqman probes on Light cycler^® Real-Time qPCR machine.

This compound requires amino- or thiol- linker with a 6-Carbon spacer arm or by substituting any thymidine with 5-C6-Amino-2'-deoxythymidine for internal labeling, a double HPLC purification is required.

Contact your Account Representative for pricing on Dyomics® dye labeled oligonucleotides. For additional information, please Contact us.

A wide variety of modifications can be incorporated into an oligonucleotide at the time of synthesis, using aspecialized phosphoramidite chemistries for labeling. However, certain modifications require the use of post-synthetic conjugation strategies in which a primary amine modified oligonucleotide is covalently attached to a dye via NHS ester chemistry. Other cross-linking chemistries are also available depending on particular project specifications. Single HPLC purification is recommended when using phosphoramidite oligo chemistry, otherwise dual HPLC is required for post-synthetic labeling.

Due to the chemistry of many modifications, yield will be approximately 40 - 60 % less than a standard oligo.Please see our yield chart for details.

Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields are determined using UV absorbance at OD₂₆₀ In addition, we perform QC methods tailored to specific modifications, such as OD ratio measurement where appropriate.

For additional information, please Contact us