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  Custom DNA Synthesis--"FASTEST services in the industry"
   
 
   
 
The use of antisense oligonucleotides (ASO’s)  was first described over twenty years ago as a method to arrest  gene expression levels both in vitro and in vivo where single-stranded DNA or RNA, in antisense configuration to a target sense mRNA. The strategies in the  design and chemistry of antisense compounds have become an effective  tool in molecular biology basic research, genomics, proteomics, target screening/validation and drug discovery.

BSI currently offer antisense oligos synthesized using DNA, 2'-O-Methyl* RNA, C-Propyne*, RNA, or LNA* bases with either phosphorothioate or unmodified internucleotide bonds.
 

Features
 
No set up fee
Deprotected, desalted, lyophilized
Purification by PAGE or RP-HPLC upon request
QC analysis by MALDI-TOF Mass spec, HPLC or PAGE
Fast delivery with high throughput capacity
   
 
Product Pricing
Scale 100 nmole 200 nmole 400 nmole 1 µmole
DNA Base $0.5/base $0.90/base  $1.50/base  $2.00/base
Phosphorothioate Bond

Synthesis + $40

Synthesis + $40  Synthesis + $50  Synthesis + $50
RNA Base $7.00/base $9.00/base  $15.00/base $18.00/base
2'-OMe* $9.00/base $11.00/base $16.00/base $19.00/base
LNA (Lock Nucleic Acids)* $25.00/base $30.00/base $40.00/base $45.00/base
5-Methyl dC Synthesis + $45 Synthesis + $45 Synthesis + $90 Synthesis + $90


A minimum will applied for oligo <10 bases. Yields and base price may vary depending on type of antisense synthesis.

Large scale synthesis is available upon requests.


contact us at 800-227-0627 or write to us at biosyn@biosyn.com

Purifications
Product Yields RNase-Free HPLC

PAGE 

100 nmole (1 OD) $65 N/A
200 nmole (2 ODs) $65 $65 for DNA base
$150 for RNA, 2'-OMe, LNA, 5-Methyl dC
400 nmole (5 ODs) $100 Inquire
1µmole (10 ODs) $150 Inquire
An additional 1-2 days of turnaround time is required for purifications. 
click here for recommended purification and applications!
 
Propyne Analog Use Agreement
Our agreement with Glen Research who in turn has an agreement with Isis Pharmaceuticals, Inc. allows us to sell to you C-5 Propynes and G-clamps that are ultimately used for RESEARCH PURPOSES ONLY. In accordance with this agreement, we must inform you of the uses to which these products may be put, which are described below. This product is covered by patents or patents pending owned by Isis Pharmaceuticals, Inc. (“Isis”). Purchase of this product includes a limited license to use this product solely for internal research. This license specifically excludes (and you have no right to use this product for): (a) therapeutic or diagnostic applications (including products or services that incorporate this product), (b) any in vivo toxicity/safety study in support of an investigational new drug application (or foreign counterpart), (c) resale (including sale of any products or services that incorporate this product) or (d) gene functionalization activities (including products or services that incorporate data derived from gene functionalization activities) if such activities have commercial application, any and all of which require a separate license from Isis. Neither this product nor any product created through its use may be used in human clinical trials. In the event you have a separate agreement with Isis regarding this product, which explicitly states that the foregoing is not applicable to you, your use of this product will be governed by the terms of such agreement. In no event does the limited license included with the purchase of this product expand or alter the scope of the license granted pursuant to such agreement.
*License Agreement: Locked-nucleic Acid (LNA) phosphoramidites are protected by EP Pat No. 1013661, US Pat No. 6,268,490 and foreign applications and patents owned by Exiqon A/S. Products are made and sold under a license from Exiqon A/S. Products are for research purposes only. Products may not be used for diagnostic, clinical, commercial or other use, including use in humans. There is no implied license for commercial use, including contract research, with respect to the products and a license must be obtained directly from Exiqon A/S for such use."

 

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