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Home » Antibody and Immunochemistry » Polyclonal Antibody Services » Select By Host Animals » Guinea Pig Polyclonal Antibody
Guniea Pig Polyclonal Antibody

Custom Guinea Pig Polyclonal Antibody Services

Bio-Synthesis (BSI) provides polyclonal antibody production using guinea pigs in its USDA-licensed facility using pathogen-free, VAF Dunkin Hartley Female guinea pigs. We can produce polyclonal antibodies with a wide variety of antigens, from bacteria, viruses, fungi, fusion proteins, synthetic peptides, and plasmid DNA, as well as a variety of other proteins.

Typical antigen requirement for guinea pig antibody production is 2 mg conjugated peptide/animal and 1 mg protein antigen/animal. However, amounts may vary due to antigen availability and additional antigen is required for affinity purification.

Three production schedules are provided for our guinea pig antibody services. We can produce polyclonal antibodies in a few to several hundred guinea pigs per run, using our standard protocol or by a customer-designed protocol. We also supply polyclonal production from other species, including goat, sheep, chicken, rat, mouse, and rabbit. Additional animals are available for all service packages.

BSI supplies a Polyclonal Antibody Information Sheet with each inquiry to assist in meeting our customer specifications.

Guinea Pig Polyclonal Antibody Service Packages

All of our antibody packages can be prolonged with an additional immunization every 28 days which includes an additional bleed 10 days after the immunization. Production extension is an additional charge of animal maintenance fee.

Request a quote or contact us for your Guinea Pig Polyclonal Antibody packages.
Service Descriptions Antigen Packages   Purification
 
Peptide
User
Supplied
Conjugation Affinity IgY A
FS-PE-GP090 First Class Standard (Guinea Pigs) Antibody Package: peptide synthesis >80%, conjugation, 70-90 days √   √      
FP-PE-GP120A First Class Plus A (Guinea Pigs) Antibody Package: peptide synthesis >80%, conjugation, protein A purification, 90-120 days √   √     √
FP-PE-GP120I First Class Plus I (Guinea Pigs) Antibody Package: peptide synthesis >90%, conjugation, peptide affinity purification 90-120 days √   √ √    
SS-PE-GP090 Super Class Standard (Guinea Pigs) Antibody Package: peptide synthesis >90%, conjugation, 70-90 days √   √      
SP-PE-GP120A Super Class Plus A (Guinea Pigs) Antibody Package: peptide synthesis >90%, conjugation, serum production, protein A purification, 90-120 days √   √     √
SP-PE-GP120I Super Class Plus I (Guinea Pigs) Antibody Package: peptide synthesis >90%, conjugation, peptide affinity purification, 90-120 days √   √ √    
US-PR-GP090 User-Supplied Protein (Guinea Pigs) Antibody Package, 70-90 days   √        
US-PR-GP90A User-Supplied Protein A (Guinea Pig) Antibody Package, protein A purification, 70-90 days   √       √
US-PE-GP120I User-Supplied Peptide Immunospecific (Guinea Pig) Antibody Package, peptide affinity, 90-120 days   √ Optional √    
US-PR-GP120I User-Supplied Protein Immunospecific (Guinea Pig) Antibody Package, immunoaffinity, 90-120 days   √   √    
Three production schedules are available for each Guinea Pig Polyclonal Antibody package along with its descriptions.

Available Production Time Frame:
  • 70-90 days
  • 84-120 days
Custom Guinea Pig Polyclonal Antibody Services
First Class Standard Peptide Antibody Package (Guinea Pig)
Package: FS-PE-GP090 (70 Days Immunization Protocol) Time Frame 70-90 days and delivery
Peptide Synthesis > 80% (<20 a.a. 15 mg) ~1 ml control serum per guinea pig
KLH/BSA conjugation via Cysteine added on C- or N-term of peptide up to 12 ml crude antiserum
2 guinea pigs, 2 control serum (1/animal), 2 initial inoculations (1/animal), 8 boosters (4/animal), 6 collection serum (3/animal) 5 mg of unconjugated peptide
ELISA titer assay on collection serum MS, HPLC of peptide, ELISA reports
  See immunization and delivery schedule.
 
First Class Plus Protein A Column Purified Peptide Antibody Package (Guinea Pig)
Package: FP-PE-GP120A (84 Days Immunization Protocol) Time Frame 90-120 days and delivery
Peptide Synthesis > 80% (<20 a.a. 15 mg) ~1 ml control serum per guinea pig
KLH/BSA conjugation via Cysteine added on C- or N-term of peptide up to 2 mg protein A purified antibodies
2 guinea pigs, 2 control serum (1/animal), 2 initial inoculations (1/animal), 10 boosters (5/animal), 8 collection serum (4/animal) 5 mg of unconjugated peptide
Protein A purification MS, HPLC of peptide, ELISA reports
ELISA titer assay on collection serum and protein A purified samples Electronic image of SDS analysis
SDS PAGE analysis See immunization and delivery schedule.
 
First Class Plus Immunoaffinity Purified Peptide Antibody Package (Guinea Pig)
Package: FP-PE-GP120I (84 Days Immunization Protocol) Time Frame 90-120 days and delivery
Peptide Synthesis > 80% (<20 a.a. 15 mg) ~1 ml control serum per guinea pig
KLH/BSA conjugation via Cysteine added on C- or N-term of peptide up to 2 mg peptide affinity-purified antibodies
2 guinea pigs, 2 control serum (1/animal), 2 initial inoculations (1/animal), 10 boosters (5/animal), 8 collection serum (4/animal) 5 mg of unconjugated peptide
Peptide immobilization on sepharose beads MS, HPLC of peptide, ELISA reports
Loading of the antiserum on the affinity column and elution of bound antibodies Electronic image of SDS analysis
ELISA titer assay on crude collection serum and peptide immunopurified antibodies See immunization and delivery schedule.
 
Super Class Package
Super Class Standard Peptide Antibody Package (Guinea Pig)
Package: SS-PE-GP090 (70 Days Immunization Protocol) Time Frame 70-90 days and delivery
Peptide Synthesis > 90% (<20 a.a. 15 mg) ~1 ml control serum per guinea pig
KLH/BSA conjugation via Cysteine added on C- or N-term of peptide up to 12 ml crude antiserum
2 guinea pigs, 2 control serum (1/animal), 2 initial inoculations (1/animal), 8 boosters (4/animal), 6 collection serum (3/animal) 5 mg of unconjugated peptide
ELISA titer assay on collection serum MS, HPLC of peptide, ELISA reports
  See immunization and delivery schedule
 
Super Class Plus Protein A Column Purified Peptide Antibody Package (Guinea Pig)
Package: SP-PE-GP120A (84 Days Immunization Protocol) Time Frame 90-120 days and delivery
Peptide Synthesis > 90% (<20 a.a. 15 mg) ~1 ml control serum per guinea pig
KLH/BSA conjugation via Cysteine added on C- or N-term of peptide up to 2 mg protein A purified antibodies
2 guinea pigs, 2 control serum (1/animal), 2 initial inoculations (1/animal), 10 boosters (5/animal), 8 collection serum (4/animal) 5 mg of unconjugated peptide
Protein A purification MS, HPLC of peptide, ELISA reports
ELISA titer assay on collection serum and protein A puriified sample Electronic image of SDS analysis
SDS PAGE analysis See immunization and delivery schedule.
 
Super Class Plus Immunoaffinity Purified Peptide Antibody Package (Guinea Pig)
Package: SP-PE-GP120I (84 Days Immunization Protocol) Time Frame 90-120 days and delivery
Peptide Synthesis > 90% (<20 a.a. 15 mg) ~1 ml control serum per guinea pig
KLH/BSA conjugation via Cysteine added on C- or N-term of peptide up to 2 mgs peptide affinity-purified antibodies
2 guinea pigs, 2 control serum (1/animal), 2 initial inoculations (1/animal), 10 boosters (5/animal), 8 collection serum (4/animal) 5 mg of unconjugated peptide
Peptide immobilization on sepharose beads MS, HPLC of peptide, ELISA reports
Loading of the antiserum on the affinity column and elution of bound antibodies Electronic image of SDS analysis
ELISA titer assay on crude collection serum and peptide immunopurified antibodies See immunization and delivery schedule.
SDS PAGE analysis  
 
User-Supplied Immunogen - Peptide/Recombinant and Fusion Protein
User-Supplied Protein Antibody Package (Guinea Pig)
Package: US-PR-GP90 (70 Days Immunization Protocol) Time Frame 70-90 days and delivery
Conjugation to carrier (optional, additional fee) ~1 ml control serum per guinea pig
2 guinea pigs, 2 control serum (1/animal), 2 initial inoculations (1/animal), 8 boosters (4/animal), 6 collection serum (3/animal) up to 12 ml crude antiserum
ELISA titer assay of collection serum ELISA report
  See immunization and delivery schedule.
Antigen requirement: 1.5-3 mgs of user-supplied protein. The concentration should be 1 mg/ml is a good target range, minimum >0.5 mg/ml in PBS buffered solution pH 7.4.
See details on antigen preparation
 
User-Supplied Protein A Purified Antibody Package (Guinea Pig)
Package: US-PR-GP90A (70 Days Immunization Protocol) Time Frame 70-90 days and delivery
Conjugation to carrier (optional, additional fee) ~1 ml control serum per gnea pig
2 guinea pigs, 2 control serum (1/animal), 2 initial inoculations (1/animal), 8 boosters (4/animal), 6 collection serum (3/animal) Protein A-purified antibodies on all serum (up to 12 ml)
Protein A purification ELISA report
ELISA titer assay of collection serum and protein A purified samples Electronic image of SDS analysis
SDS-PAGE analysis See immunization and delivery schedule.
Antigen requirement: 1.5-3 mgs of user-supplied protein. The concentration should be 1 mg/ml is a good target range, minimum >0.5 mg/ml in PBS buffered solution pH 7.4.
See details on antigen preparation
 
User-Supplied Peptide Immunospecific Purified Antibody Package (Guinea Pig)
Package: US-PE-GP120I (84 Days Immunization Protocol) Time Frame 90-120 days and delivery
Conjugation to carrier (optional, additional fee) ~1 ml control serum per guinea pig
2 guinea pigs, 2 control serum (1/animal), 2 initial inoculations (1/animal), 10 boosters (5/animal), 8 collection serum (4/animal) Immunospecific affinity-purified antibodies on all serum (up to 16 ml)
Peptide immobilization on sepharose beads Peptide-bounded affinity column
Loading of the antiserum on the affinity column and elution of bound antibodies ELISA report
ELISA titer assay on crude collection serum and peptide immunopurified antibodies Electronic image of SDS analysis
SDS-PAGE analysis See immunization and delivery schedule.
Antigen requirement: 8-10 mgs of user-supplied peptide (2-3 mgs for immunization, 5 mgs for purification) The concentration should be 1 mg/ml is a good target range, minimum >0.5 mg/ml in PBS buffered solution pH 7.4. Additional requirements may apply, please contact us for details.
See details on antigen preparation
 
User-Supplied Protein Immunospecific Purified Antibody Package (Guinea Pig)
Package: US-PR-GP120I (84 Days Immunization Protocol) Time Frame 90-120 days and delivery
Protein analysis by SDS-PAGE ~1 ml control serum per guinea pig
2 guinea pigs, 2 control serum (1/animal), 2 initial inoculations (1/animal), 10 boosters (5/animal), 8 collection serum (4/animal) Immunospecific affinity-purified antibodies on all serum (up to 16 ml)
Protein conjugated to affinity resin (protein dialysis in PBS) Protein-bounded affinity column
Loading of the antiserum on the affinity column and elution of bound antibodies ELISA report
ELISA titer assay of crude collection serum and protein immunopurified antibodies Electronic image of SDS analysis
SDS-PAGE analysis See immunization and delivery schedule.
Antigen requirement: 8-10 mgs of user-supplied protein (2-3 mgs for immunization, 5 mgs for purification) The concentration should be 1 mg/ml is a good target range, minimum >0.5 mg/ml in PBS buffered solution pH 7.4.
See details on antigen preparation
Custom Polyclonal Guinea Pig AntibodyGuinea Pig Polyclonal Antibody Immunization Protocol

We offer two standard custom antibody protocols (combinations of hosts and protocol time frame) From peptide design, synthesis, and conjugation, to antibody generation and purification, Bio-Synthesis offers a complete package to meet your protein detection needs. User-design production protocol also available and can also be modified from existing package.

Serum Production Specification:
Immunization schedule listed below are approximate (± 2 days).
  • Two VAF Dunkin Hartley female guinea pigs will be utilized for each MAP-peptide, conjugated- peptide immunogen, or protein immunogen packages
  • Total material required for injection for the program below is  600-800 ug protein/animal, 2.5 mg conjugated peptide/animal . If we are conjugating your protein we assume a 20% loss of protein during conjugation. Total amount of serum expected from a guinea pig on the program below is approximately ~3-8 mL.
  • Immunogen is emulsified in Freund's Complete Adjuvant (FCA) for initial injections. Freund's
    Incomplete Adjuvant (FIA) is used for all subsequent injections (boosts).
  • Continuation of project past our standard immunization schedule is offered on a month to month basis. The standard extension protocol includes two boosters, one serum collection, and maintenance for one month. Terminal bleeds may be obtained at any time yielding approximately ~15-20 ml/guinea pig of additional serum taken upon customer request.
 
Guinea Pig Polyclonal Antibody Production Protocol
Program Development of Guinea Pig Polyclonal Antibody: 70-90 Days
Procedure Protocol Days* Description
Phase I - Antigen design, synthesis and preparation
Antigen Preparation ~ 5 - 10 days Peptide synthesis and purification (if required) and antigen conjugation
Phase II - Immunization Schedule
Control serum collection
Initial Inoculation
Day 0
VAF Dunkin Hartley Female and Control serum (~1 ml serum/guinea pig)
Initial Inoculation with 250 µg peptide or 200 µg protein per host with FCA in SQ 4 sites
1st Booster Day 14 Boost with 250 µg peptide or 100 µg protein per host with FIA in SQ 4 sites
2nd Booster Day 28 Boost with 250 µg peptide or 100 µg protein per host with FIA in SQ 4 sites
1st Serum Collection Day 42 Week 6 Collection serum ~1-2 ml/guinea pig
3rd Booster Boost with 250 µg peptide or 100 µg protein per host with FIA in SQ 4 sites
2nd Serum Collection

4th Booster

ELISA 		Day 56-63 Boost with 250 µg peptide or 100 µg protein per host with FIA in SQ 4 sites

Week 8 Collection serum ~1-2 ml/guinea pig

ELISA assay of control and week 8 collection serum
1st Shipment

Project Completion Verification 		Day 63-64 Shipping of control, week 6 and 8 collection serum

Verify disposition of guinea pigs, extension or termination
3rd Serum Collection Day 70 Week 10 Collection serum ~1-2 ml/guinea pig
Final Shipment Day 75 Shipping of week 10 collection serum
Instruction Due Date Day 84 Termination of animals if instruction not received
 
Guinea Pig Polyclonal Antibody Production Protocol
Program Development of Guinea Pig Polyclonal Antibody: 90-120 Days
Procedure Protocol Days* Description
Phase I - Antigen design, synthesis and preparation
Antigen Preparation ~ 5 - 10 days Peptide synthesis and purification (if required) and antigen conjugation
Phase II - Immunization Schedule
Control serum collection
Initial Inoculation
Day 0
VAF Dunkin Hartley Female and Control serum (~1 ml serum/guinea pig)
Initial Inoculation with 250 µg peptide or 200 µg protein per host with FCA in SQ 4 sites
1st Booster Day 14 Boost with 250 µg peptide or 100 µg protein per host with FIA in SQ 4 sites
2nd Booster Day 28 Boost with 250 µg peptide or 100 µg protein with FIA in SQ 4 sites
1st Serum Collection Day 42 Week 6 Collection serum ~1-2 ml/guinea pig
3rd Booster Boost with 250 µg peptide or 100 µg protein with FIA in SQ 4 sites
2nd Serum Collection

4th Booster 		Day 56 Week 8 Collection serum ~1-2 ml/guinea pig

Boost with 250 µg peptide or 100 µg protein per host with FIA in SQ 4 sites
3rd Serum Collection

5th Booster

ELISA Titer Assay 		Day 70-75 Week 10 Collection serum ~1-2 ml/guinea pig

Boost with 250 µg peptide or 100 µg protein per host with FIA in SQ 4 sites

ELISA assay on control, week 6, 8 and 10 collection serum
1st Shipment

Project Completion Verification 		Day 77 Shipping of control, 6, 8 and 10 collection serum and ELISA report

Verify disposition of guinea pigs, extension or termination
4th Serum Collection Day 84 Week 12 Collection serum ~1-2 ml/guinea pig
Phase III - post production sample processing

Instruction Due Date 		Day 98 Preparation of phase III sample processing

Termination of animals if instruction for extension or termination not received
Phase II - Immunization Schedule
Serum Processing ~ 2 weeks affinity column preparation, purification and/or labeling
ELISA and/or SDS Analysis ~ 1 weeks Analysis of purified sample
Final Shipment Day 108 Shipping of purified sample, unpurified week 12 serum and documents
 
 
Links and Resources

Forms:
Order/Quote Page

Additional Phospho-Specific Antibody Information:
Phospho-Specific Life-Cycle Illustration

Sci Apps:
Peptide Property Calculator
Peptide Design Library
Oligo Designer

Useful Links
Reference/Citing:
1.
Different prelamin A forms accumulate in human fibroblasts: a study in experimental models and progeria
2.
1,25-DIHYDROXYVITAMIN D3-INDUCED GENES IN OSTEOBLASTS: UNCOVERING NEW FUNCTIONS FOR MENINGIOMA 1 AND SEMAPHORIN 3B IN SKELETAL PHYSIOLOGY
3.
Fetal Death in Mice Lacking 5α-Reductase Type 1 Caused by Estrogen Excess
4.
A splicing variant of a death domain protein that is regulated by a mitogen-activated kinase is a substrate for c-Jun N-terminal kinase in the human central nervous system
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