Fastype™ Molecular Detection Kits for
Human Infectious Diseases
Bio-Synthesis Inc. is proud to introduce the Fastype™ Conjunctivitis Adenovirus
and Chlamydia DNA PCR-SSP Detection Kits to the molecular infectious diseases testing
labs. The molecular test is also known as Nucleic Acid Amplification Test, it is
based on the amplification of the present of target DNA in the specimen rather than
the conventional method of cell or bacteria culture.
The primary features of the Fastype™ kits are listed as following:
- All primers, dNTPs and PCR buffer are pre-aliquot and lyophilized
in high-quality PCR thermal cycle 0.2ml color coded micro strip tubes. Genomic DNA
template of Adenovirus and Chlamydia are also added in the Positive Control Kits
respectively for monitoring of PCR condition and the comparison of final amplified
fragment.
- Fastype™ SSP Kits reduce the preparation time to less then
10 minutes.
- Lyophilized primers, dNTPs and PCR buffer are more stable than
in liquid form under ambient condition, and the final lyophilized red dot (reagents)
in the bottom of each PCR strip tube can distinguish clearly from the regular PCR
strip tubes.
- The PCR Protocol for all Fastype™ System is kept the same regardless
of the kit type.
- The PCR protocol, Certificate of Product and gel loading dye
are also included in the shipment, and we have strong technical support.
Adenoviruses
Adenoviruses are a group of viruses that infect the membrane of the eyes, respiratory
tract, the intestines, and urinary tract, they are highly contagious. Pinkeye (Conjunctivitis)
always occurs in school children when the virus cause inflammation of the conjunctiva,
and they also account for about 10% of acute respiratory infections in children
and are a frequent cause of diarrhea. The three testing kits in this category are
described as following:
|
Cat Number
|
Product Name
|
Descriptions
|
|
20 – AdTu – FA
|
Fastype™ Adenovirus PCR – SSP Detection Kit A
|
It is a single PCR – SSP reaction kit that detects Adenovirus DNA from infected
specimens. This is the first round of the PCR amplification, and the amplified end
product is 1,004 bp (base pair). The final lyophilized detection reagents are lyophilized
in amber color PCR strip tubes
|
|
20 – nAdTu – FB
|
Fastype™ Adenovirus Nested PCR – SSP Detection Kit B
|
It is a single PCR – SSP reaction kit, it is used as a second round PCR to amplify
20 – AdTu – FA amplicon. The final amplified product is 956 bp, and the products
are prepared in blue color PCR strip tubes.
|
|
20 – AdTu – FC+
|
Fastype™ Adenovirus PCR – SSP Positive Control Kit
|
It is a single PCR – SSP reaction kit, Adenovirus genomic DNA template is added
inside the PCR reaction tube to serve as a positive control and to monitor the PCR
condition. The PCR fragment should yield a 1,004 bp band, and the kit is kept in
clear PCR strip tubes.
|
|
Chlamydia
Chlamydia is a disease caused by the bacteria Chlamydia trachomatis, it is one of
the most common sexually transmitted diseases in the U. S., thus, it is often called
“the silent epidemic”, because infections are so prevalent yet many people do not
know that they are infected. The advantage of molecular DNA – SSP detecting method
is that generally more sensitive, specific and speedy than conventional culture,
and can therefore identify more positive specimens. The DNA – SSP kits are briefly
described as following:
|
Cat Number
|
Product Name
|
Descriptions
|
|
20 – Ch1/Ch2 – FCh
|
Fastype™ Chlamydia PCR – SSP Detection Kit
|
It is a single PCR – SSP reaction kit, detects omp2 gene Chlamydiaceae Species.
The final amplified end product is 587 bp, and all components are lyophilized in
the green color PCR strip tube.
|
|
20 – Ch1/Ch2 – FChC+,
|
Fastype™ Chlamydia PCR – SSP Positive Control Kit
|
It is a single PCR – SSP reaction kit, Chlamydia genomic DNA template is added inside
the PCR – reaction tube to serves as a positive control standard for Chlamydia PCR
– SSP detection kit. The final amplified fragment is 587 bp, and the kit is prepared
in red color PCR strip tube.
|
|
References:
- Suresh Dalapathy, Therese K. Lily, Sara Roy, and Hajib N. Madhavan.
Development and use of nested polymerase chain reaction (PCR) for the Detection
of Adenovirus from conjunctivitis specimens. J. Clinical Virology 11:77 – 84, 1998.
- Hartley, J. C., S. Kaye, S. Stevenson, J. Bennett, and G. Ridgeway.
PCR detection and molecular identification of Chlamydiaceae species. J of Clinical
Microbiology, 39, 9: 3072 – 3079, 2001.
- Meijer, A., J. Kwakkel, A. de Vries, L M. Schouls, and J. M.
Ossewaarde. Species identification of Chlamydia isolates by analyzing restriction
fragment length polymorphism of the 16S – 23S rRNA spacer region. J of Clinical
Microbiology, 35, 5:1179 – 1183, 1997.