Modification Specific Polyclonal Antibody Services
 
Modification Specific Polyclonal Antibody Services

Detection of Posttranslational Modification

Posttranslational Modification (PTM) is a key component that contributes to the diversity of the biological activities and affecting a wide range of protein characterization or cellular functions. Antisera raised against these protein modifications has shown to be an useful tools in the study of cell signaling, protein structure, function, localization, activity, maturation, disease diagnostic, etc.

While protein phosphorylation and methylation is considered the most common posttranslational modification, more than 200 different covalently attached modifications targeting most proteinogenic amino acids are known today. Bio-Synthesis has been an expert in providing custom-made modification specific peptides as antigens for antiserum production. All antibodies against posttranslational modifications are double purified at Bio-Synthesis against the sequence and the modified peptide to ensure specificity.

Contact us about your project and we will reply with a quote along with detailed information regarding your custom Modification Specific Polyclonal Antibody Production!

Below is our custom-made posttranslational modification specific antibody development program, should you not find the approach or modification that suits your needs, you have the option to develop a customized host species and immunization schedule to meet your protein detection requirement. For project workflow, click on Our Approach tab! Additional services such as protein analysis and assay development also available.

Request a quote or contact us for your Modification Specific Polyclonal Antibody packages.
 
OUR SERVICE
Synthesis of modified and unmodified peptide
up to 20 aa, >90% pure, 15 mgs each
Conjugation: KLH/BSA through Cysteine
2 rabbits immunization, 10 inoculations
6 Serum collection bleeds 70 days protocol
Two affinity columns
Double peptide specific affinity purification
using modified and unmodified-peptide affinity
column
ELISA against modified and unmodified peptide
YOU RECEIVE
~ 2 mls of control sera
3-5 mgs of free unmodified and modified
peptides
1-30 mgs of affinity-purified antibodies (available
in large scale)
Remaining unpurified anti-sera
Two Affinity purification column
ELISA reports, additional certificate of analysis
Modification Type Catalog Number Price
Phospho-specific Anti-Peptide Polyclonal Antibodies    
Phospho-specific (Serine, Threonine, Tyrosine) FCC-PE-PHOS-RA120II $2,500
Phospho-specific (Serine, Threonine, Tyrosine) SCC-PE-PHOS-RA120II $3,000
Phospho-specific (Serine, Threonine, Tyrosine) SCW-PE-PHOS-120II $3,800
Phospho-specific (Serine, Threonine, Tyrosine) SCT-PE-PHOS-RA120IIA $3,200
Methyl-specific Anti-peptide Polyclonal Antibodies    
Mono-Methyl (Lysine) Specific FCC-MSPAB001 Inquire
Di-Methyl (Lysine) Specific FCC-MSPAB002 Inquire
Tri-Methyl (Lysine) Specific polyclonal antibody FCC-MSPAB003 Inquire
Mono-Methyl (Arginine) Specific polyclonal antibody FCC-MSPAB004 Inquire
Di-Methyl (Symmetric Arginine) Specific polyclonal antibody FCC-MSPAB005 Inquire
Di-Methyl (Asymmetric Arginine) Specific polyclonal antibody FCC-MSPAB006 Inquire
Aceytylation-specific Anti-Peptide Polyclonal Antibodies    
Epsilon-acetyl lysine FCC-MSPAB007 Inquire
N-terminal Acetylation FCC-MSPAB008 Inquire
Nitrotyrosine-specific Anti-Peptide Polyclonal Antibodies    
Nitro-tyrosine FCC-MSPAB009 Inquire
Other Posttranslational Modification Specific Peptide Polyclonal Antibody    
Formylation, Myristoylation, Palmitoylation, Steroylation, Hydroxyproline, Ubiquitination, SUMOylation, Neddynation (branched peptides), Sulphatation, Citrullination, Met/Cys Sulfoxide, Lipidation FCC-MSPAB010 Inquire
Anti-neoepitope Specific Anti-Peptide Polyclonal Antibody for Proteoytic Processing
   
N-terminal neo-epitope FCC-MSPAB011 Inquire
C-terminal neo-epitope FCC-MSPAB012 Inquire
Modification Specific Peptide Co-Immunization    
Modification Peptide Co-Immunization: a mix of two peptide for immunization FCC-MSPAB013 Inquire

Below is our custom-made posttranslational modification specific antibody development program. Should you not find the approach that suits your needs, you have the option to develope a customized host species and immunization schedule to meet your protein detection needs.



Program Development of Rabbit Polyclonal Antibody: 70-90 Days
Project Assignments
Upon signing of CDA agreement, BSI will immediately assign a dedicated project manager to oversee and coordinate the entire production process.
 
Peptide Antigen Selection
Free peptide antigen selection services using our proprietary Antigen Identifier Designing tool.
 
Peptide Synthesis
20 mgs of unmodified and modified peptide synthesis carrying the modification to mimic posttranslational modification.
 
Peptide Carrier Conjugation
Conjugation of Peptide and carrier protein of your choice.
 
Immunization
70 day immunization protocol with 2 hosts, 2 control bleed, 10 immunization (5/animal), 6 serum collection (3/animal)
Immune Response Monitoring
ELISA assay to identify the best responding animal host.
 
Identifying the best responding Host
Using ELISA assay as a indirect method to select the best responding host
 
Anti-Sera Purification
Antiserum with best responding host will undergo double affinity purification with modified and unmodified peptide
 
Antibody Characterization
Purified antiserum validated with vigorous application testing such as ELISA, Western Blot, SDS-PAGE, etc.
 
Product Dispatch

You Receive:

~ 2 mls of control sera
1-3 mgs of affinity-purified antibodies (available in large
scale)
Remaining unpurified antisera
Modified and Unmodified affinity column
MS, HPLC of peptide and ELISA reports
Electronic image of SDS analysis

Ordering Services
To place an order or request a quotation, please proceed to our custom Order/Quote page or Contact Us for any other questions.
Order can be placed with either a PO (Purchase Order) or credit card. You may place order online using our secure online ordering system. Our customer service representatives are available 24 hours a day, Monday through Friday, to assist you.
   
We accept POs and major credit cards ( ). Your credit card will be billed under "Bio-Synthesis, Inc." Please complete and return our Business Credit Application. For international orders, we must apply the full charge at the time of the order is placed. In the unlikely event that any given order cannot be filled, our guarantee will take the form of a full refund.