Monoclonal Antibody Services

Bio-Synthesis offers custom monoclonal antibodies (mAb) using synthetic peptide or protein antigen with guaranteed ELISA positive clones when antigens are designed, produced and prepared by Bio-Synthesis. The monoclonal antibodies are performed under leading-edge technology in the accredited facility, making the development of monoclonal antibodies fast and affordable for your antibody diagnostic and therapeutic studies. Bio-Synthesis monoclonal antibody services provide different phases, allowing you the flexibility to control every hybridoma development stage. If any phase should meet limited success, the project can be terminated without incurring the expense of the whole program cycle.

Guarantees

We guarantee two positive clones for monoclonal antibodies development if peptide antigens were designed, synthesized, and conjugated by Bio-Synthesis. For any protein antigen (>90% purity), we guarantee two positive clones with positive Western blot results.

Service Highlights

All of our monoclonal antibody development packages include:

• Free peptide design
• Immunization of 5 BALB/c mice
• Screening 3x by ELISA
• Western Blot and/or ICC screening in some package(s)
• Fusion, subclone 2x, expansion and cryopreservation
• Three cell line stabilization
• Isotyping to confirm Ig class
• Cell line banking for 1 year

Quality Controls Measures

The following quality control measures are employed to create the highest quality monoclonal antibodies commercially available:

• ELISA against the antigen
• SDS/PAGE
• Immunocytochemistry (ICC)
• HPLC
• Western Blot

Additional functional analysis can be performed upon client's request using:

• Flow cytometry analysis (FC)
• Immunohistochemistry (IHC)
• Immunoprecipitation (IP)

Standard Protein Monoclonal Antibody Package: mAb1000

Package: mAb1000	Deliverable (4-6 months)
Immunization and ELISA screening	3 positive hybridoma clones
Fusion plating and screening by ELISA	Two vials of cell culture for each clone
Subcloning 2x, ELISA screening, expansion, cryopreservation	2 ml of culture supernatant
Final clone isotyping	ELISA data & isotyping report

Premium Protein Monoclonal Antibody Package: mAb2000

Package: mAb2000	Deliverable (4-6 months)
Immunization and ELISA screening	3 positive hybridoma clones
Fusion plating and screening by ELISA	Two vials of cell culture for each clone
Subcloning 2x, ELISA screening, expansion, cryopreservation	2 ml of culture supernatant
Screen by ELISA and ICC or WB after 1st and 2nd subcloning	ELISA data, ICC or WB report
Final clone isotyping	Isotyping report

Standard Peptide Monoclonal Antibody Package: mAb3000

Package: mAb3000	Deliverable (4-6 months)
Peptide synthesis >90% (<25 a.a.)	3 positive hybridoma clones
KLH conjugation via Cysteine	Two vials of cell culture for each clone
Immunization and ELISA screening	2 ml of culture supernatants
Fusion plating and screening by ELISA	5 mg of unconjugated peptide
Subcloning 2x, ELISA screening, expansion, cryopreservation	ELISA data
Final clone isotyping	Isotyping report

Premium Peptide Monoclonal Antibody Package: mAb4000

Package: mAb4000	Deliverable (4-6 months)
Peptide synthesis >90% (<25 a.a.)	3 positive hybridoma clones
KLH conjugation via Cysteine	Two vials of cell culture for each clone
Immunization and ELISA screening	2 ml of culture supernatants
Fusion plating and screening by ELISA	5 mg of unconjugated peptide
Subcloning 2x, expansion, cryopreservation	ELISA report
Screen by ELISA and ICC or WB after 1st and 2nd subcloning	ICC or WB report
Final clone isotyping	Isotyping report

Bio-Synthesis offers a variety of hybridoma development and monoclonal antibody production services. Customer personalize packages are also available. Contact our experienced customer representatives to help you select or design the best service for your specific needs.

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