Sample Preparation Products

For the rapid preparation of high quality DNA from whole blood, buffy coat, plasma and serum } Input volumes up to 150 µL No phenol required for DNA isolation Extract DNA from whole blood, buffy coat, plasma and serum Fast and convenient spin column protocol

Bio-Synthesis's Blood Genomic DNA Isolation Micro Kit is designed for the rapid preparation of genomic DNA from up to 1500 µL of whole blood, buffy coat, plasma and serum. Purification is based on spin column chromatography using Bio-Synthesis's proprietary resin as the separation matrix. Briefly, the sample is lysed and the released genomic DNA is bound to Bio-Synthesis's column in the presence of Binding Solution (BIND). Under these conditions only the genomic DNA is bound to Bio-Synthesis's resin while most of the contaminating RNA and cellular proteinaceous components are removed in the flowthrough. The bound DNA is then washed to remove any remaining impurities (WASH). Lastly, the purified genomic DNA is eluted into 100 - 200 µL of the provided Elution Buffer or water (ELUTE). Please see the procedure flowchart to the right. The Blood Genomic DNA Isolation Micro Kit allows for the isolation of genomic DNA from the blood of various species, including humans. Typical yields of genomic DNA will vary depending on the cell density of the blood sample. Preparation time for a single sample is less than 30 minutes, and each kit contains sufficient materials for 50 preparations. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications including PCR and Southern Blot analysis.

FEATURES AND BENEFITS No Phenol-Chloroform extraction or alcohol precipitation - Isolate genomic DNA without the use of harmful chemicals Fast and easy processing - Rapid spin-column format allows for the processing of multiple samples in 30 minutes. Isolate genomic DNA from various inputs - Isolate DNA from various blood inputs including plasma, whole blood, buffy coat and serum Recovered genomic DNA is suitable for downstream applications - Purified genomic DNA is fully compatible with restriction enzyme digestions, Southern Blot and PCR analysis. High quality DNA with no RNA contamination - No contamination or degradation of genomic DNA isolated with the Blood Genomic DNA Isolation Micro Kit is observed.

APPLICATIONS Genomic DNA isolated using the Blood Genomic DNA Isolation Micro Kit is well-suited to a wide range of downstream applications including: qPCR PCR Sequencing Restriction Digests Southern blotting

Figure 1. High Yields of Genomic DNA Isolated from Whole Blood. Genomic DNA was isolated from two different 150 µL whole blood samples using Bio-Synthesis's Blood Genomic DNA Isolation Micro Kit and a leading competitor's kit. Following isolation, 10 µL from 200 µL elution was loaded on 1% TAE agarose gel. Bio-Synthesis's Blood Genomic DNA Isolation Micro Kit demonstrated a better DNA yield than the leading competitor's kit. Lane M: Bio-Synthesis's HighRanger 1kb DNA Ladder.

Figure 2. Purified DNA Can be Amplified in a Real-time PCR (SYBR Green) Reaction. Genomic DNA was isolated from 150 µL of whole rat blood ( Δ ) and whole rabbit blood (O) samples using Bio-Synthesis's Blood Genomic DNA Isolation Micro Kit (Blue) and a leading competitor's kit (Red). Two µL of the DNA from each 200 µL of elution was used in a real-time PCR reaction (total reaction volume of 20 µL) with 5s rDNA primers. The real-time PCR was successful in amplifying 5s gene from both the rat and rabbit blood DNA, indicating that the DNA is of a high quality and can be used in sensitive downstream applications. Furthermore, Bio-Synthesis's-isolated DNA was amplified with a lower Ct value, indicating the higher yield and purity of DNA isolated using Bio-Synthesis's kit.

Kit Specifications Maximum blood input 150 µL Average Yield (150 µL of blood) 3 to 5 µg* Time to Complete 10 Purifications 30 mins * Yield will vary depending on the type of blood processed

Blood Genomic DNA Isolation Kit Contents 1. Resuspension Buffer 2. Lysis Solution 3. Proteinase K (lyophilized) 4. Wash Solution I 5. Wash Solution II 6. Elution Buffer 7. Micro Spin Columns 8. Collection Tubes 9. Elution Tubes 10. Product Insert

Long-Term Storage: All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

Product Usage: For laboratory research use only, not for diagnostic use! Not for use in humans.

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