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Cell Lysis Reagent

Catalog#:

NB-18800

Price/Unit:

$116.00

Unit:

100 mL

For the efficient lysis of bacterial cells and the extraction of inclusion body proteins

Proprietary solution of detergents, protease-inhibitors and buffer
Efficient lysis of bacterial cells for extraction of inclusion body proteins

Bio-Synthesis's Cell Lysis Reagent is designed for the gentle and efficient lysis of bacterial cells in order to assist in the extraction of inclusion body proteins. Cell lysis is accomplished through non-ionic detergent chemical disruption in conjunction with mechanical disruption. The use of a needle and syringe during the procedure helps to reduce viscosity and facilitate purification, producing proteins that are often greater than 95% pure.

Cell Lysis Reagent does not solubilize inclusion bodies. Inclusion Bodies can be solubilized using Bio-Synthesis's Inclusion Body Solubilization Reagent (available separately). Bio-Synthesis's Inclusion Body Protein Isolation Kits can also be used as complete kits for cell disruption, inclusion body solubilisation and purification using spin column chromatography.

APPLICATIONS
Proteins purified using Bio-Synthesis's Cell Lysis Reagent can be used in a number of downstream applications including:

SDS-PAGE analysis
Western blot
ELISA
Refolding experiments
Mass spectrometry
Further purification and scale up

Figure 1: Efficient Isolation of Inclusion Body Proteins Using Bio-Synthesis's Cell Lysis Reagent and Inclusion Body Solubilization Reagent. Inclusion Bodies were extracted and solubilized following gene expression. Recovered proteins were analyzed on 12% SDS-PAGE and stained with Coomassie Brilliant Blue R-250. Numbers represent kDa sizes of protein bands

Customer-supplied Reagents and Equipment

Centrifuge
Micropipettors
Needles (20 gauge for small scale protocol and 18 gauge for large scale protocol)
Syringes (1 mL for small scale protocol, 10 mL for large scale protocol)
Sterile deionized or Milli-Q ® water

Product Usage: For laboratory research use only, not for diagnostic use! Not for use in humans.

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