Sample Preparation Products

For rapid extraction of DNA from agarose gel fragments

Fast and easy recovery of DNA from agarose gel fragments High recovery Convenient spin column format

BSI’s DNA Gel Extraction Kit enables rapid purification of DNA fragments that have been fractionated on agarose gels. The kit effectively removes gel contaminants, allows for buffer exchange and yields high quality DNA appropriate for many downstream applications.

Purification is based on spin column chromatography using BSI’s proprietary resin as the separation matrix. Breifly, the DNA of interest is run on an agarose gel and the band is then excised from the gel. Binding Solution is added to the gel slice to melt the agarose, and the released DNA is then bound to BSI’s column (BIND). Under these conditions only the DNA will bind to BSI’s resin while the agarose and other impurities are removed in the flowthrough. The bound DNA is then washed to remove any remaining impurities (WASH). Lastly, the purified DNA is eluted into 30 - 50 µL of the provided Elution Buffer or water (ELUTE). Please see the procedure flowchart to the right.

BSI’s DNA Gel Extraction Kit effectively removes gel contaminants, allows for buffer exchange and yields high quality DNA appropriate for many downstream applications. The kit is able to recover DNA ranging in size from 100 bp to 15,000 bp. Each column has a binding capacity of 25 mg, and the typical purification yield is up to 90% of the input amount.

FEATURES AND BENEFITS

• Fast and easy processing - Rapid spin-column format allows for the processing of multiple samples in 20 minutes.
• High Recovery - Recovery of DNA fragments up to 90% for inputs of > 1 mg.
• Recovered DNA is suitable for downstream applications - Purified DNA is fully compatible with restriction digestions, ligations, sequencing, PCR and other downstream applications.
• Useable with a variety of agarose percentages - Kit is compatible with agarose gel concentrations of up to 4%.

APPLICATIONS
DNA purified using BSI's DNA Gel Extraction Kit can be used in a number of downstream applications including:

• Restriction digestions
• Ligations
• Sequencing
• PCR

Figure 1. Efficient Recovery from Agarose Gels. The high yield of BSI ’s DNA Gel Extraction Kit is illustrated by purification of 1.5 µg of a 500 bp fragment from a 1% agarose gel using BSI ’s DNA Gel Extraction Kit and two other commercial kits. DNA recovery was quantified by running 300 ng (1/5th) of the input and 10 µL (1/5th) of the 50 µL DNA elution on a 1X TAE, 1% agarose gel followed by densitometry. The BSI kit shows a significantly higher efficiency compared to the competitor kits based on the average of three purifications.

Figure 2. Efficient Recovery of Large DNA Fragments. The efficient recovery of BSI ’s DNA Gel Extraction Kit is illustrated by purification of 1.5 µg of a 3,700 bp fragment from a 0.9% agarose gel using BSI ’s DNA Gel Extraction Kit (Lanes 5-7) and a competitor’s kit (Lanes 2-4). Eluted DNA was resolved on a 1X TAE, 0.9% agarose gel. Lane 1 indicates 300 ng (20%) of the input amount, while lanes 2-7 contain 10 µL (20%) of the 50 µL eluted amount. BSI ’s kit shows a higher recovery than the competitors kit.

DNA Gel Extraction Kit Contents
1.	Binding Solution
2.	Wash Solution
3	Elution Buffer
4.	Micro Spin Columns
5.	Collection Tubes
6.	Elution Tubes
7	Product Insert

Long-Term Storage: All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

Product Usage: For laboratory research use only, not for diagnostic use! Not for use in humans.