Removing endotoxins has become a pivotal step for advancing research. As contamination threatens to invalidate data, the need for endotoxin removal kits is ever growing. Bio-Synthesis now provides kits for rapid and efficient endotoxin removal from proteins and peptides. The kits are available in a convenient spin column format. The columns bind proteins of interest while the endotoxins flow through, with a protein recovery rate of over 95% of input.
This kit works by binding proteins to the spin column matrix while endotoxins and other contaminants are washed away. The purified proteins and peptides are then eluted and ready for downstream applications. A unique feature of the kit is that the endotoxins can be washed away repeatedly until desirable levels of endotoxins are obtained. Other commercially available kits employ membranes to bind the endotoxins and therefore can quickly become saturated with endotoxins, however, Bio-Synthesis's columns do not become saturated as the endotoxins are not bound on the column. In addition, no further purification steps are required with Bio-Synthesis's kit to clean-up the sample prior to use in downstream applications.
Bio-Synthesis's kit will desalt, remove detergents and allow for buffer exchange while simultaneously removing endotoxins, therefore saving considerable time and expense. The purified protein is suitable for in vivo and in vitro introduction into cells and organisms, monoclonal and polyclonal antibody generation, and other applications such as mass spectrometry, gel electrophoresis and immunoassays and western blots. Purification is based on spin column chromatography using Bio-Synthesis's proprietary resin as the separation matrix.
The endotoxin kits offer several key benefits. The protocol allows for fast and efficient processing of multiple samples in 20 to 30 minutes. Columns are provided ready-to-use out of the box. Unlike other endotoxin removal methods, there is no need for lengthy column or resin preparation.
Researchers will now be able to use their time and money more efficiently while advancing their research simultaneously.