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A wide variety of modifications can be incorporated directly during the synthesis
or after synthesis. Certain modifications (notably Digoxigenin and some fluorescent
dyes) are not available to be incorporated during synthesis and must be attached to the oligo
after synthesis using NHS ester chemistry. NHS esters react with free primary amines
and result in stable, covalent attachments. A primary amine is, therefore, added
to the oligo during synthesis to permit reaction with the desired NHS ester. The
post-synthetic chemical modifications made to an oligonucleotide by using NHS ester
modification result in lower yields than
direct incorporation of modifications during synthesis. Furthermore, all NHS ester
modifications require HPLC purification. PAGE purification is not offered for NHS
ester modifications as yields are further decreased and certain modifications can
be damaged during PAGE purification.
Don't see the modification you are interested in? Just ask — in most cases
we can accommodate your request. And if you require help in choosing the optimal
modification, you can consult the experts by contacting us.
We offer a personalized solutions to assist client cross link various molecules
and compound and solid support attachment.
Use antisense oligonucleotides for your gene silencing experiments. We hybrid designs
using various types of nucleotide analogs to increase high affinity for a successful
knockdown experiments. More....
Bio-Synthesis offers labeling of oligonucleotide or peptide/protein with redox active
compounds for electrochemical studies.
Bio-Synthesis offers metal chelator oligo incorporation of 2,2’-Dipicolylamine. This
is a versatile metal-coordinating ligand capable of forming complexes with common
metal ions including Zn2+, Ni2+, Cu2+, or Ag+. A tremendous advantage of dipicolylamine
is complete compatibility with standard DNA synthesis, cleavage and purification
protocols. Other chelating ligands may require nonstandard conditions or additional
protection and deprotection steps. This product was manufactured and developed by
Syntrix Biosystems Inc. Patents Pending. For Research Use Only.
Pyrene and perylene are fluorescent polycyclic aromatic hydrocarbons that have the
ability to form ‘excited state dimers’ known as excimers. This unstructured, long-wavelength
emission arises from the formation of a charge-transfer complex between the excited
state and the ground state of two fluorescent molecules. In Pyrene-dU and perylene-dU,
the hydrocarbon is attached at the 5 position of deoxyuridine through a triple bond
and is electronically coupled to the deoxyuridine base. This electronic coupling
of the base and the hydrocarbon makes the fluorescence sensitive to the base pairing
of the dU portion of the molecule, allowing the discrimination between perfect and
one base mismatched targets.
Photo-control, the use of ultraviolet or visible light to control a reaction, has
a number of advantages over other external stimuli:
When a photo-responsive molecule is directly attached to DNA as a receptor, photo-regulation
of the bioprocess regulated by that DNA molecule could, in principle, be achieved.
Such photo-responsive DNA could also be used as a switch in a DNA-based nano-machine.
Professor Hiroyuki Asanuma and his group at the department of Molecular Design and
Engineering of the Graduate School of Engineering of the Nagoya University (Japan)
have developed an efficient method to achieve this goal. They have attached azobenzene
to DNA and made it photo-responsive. Azobenzene is a typical photo-responsive
molecule that isomerizes from its planar trans-form to the non-planar cis-form after
UV-light irradiation with a wavelength between 300 nm and 400 nm (lmax is around
330 nm). Interestingly, the system reverts from the cis-form to the trans-form after
further irradiation with visible light (wavelength over 400 nm). This process is
completely reversible, and the azobenzene group does not decompose or induce undesirable
side reactions even on repeated trans-cis isomerization. By introducing azobenzenes
into DNA through D-threoninol as a linker, Asanuma and co-workers succeeded in achieving