Oligo Designer 1.51

Sequence Accepted Input
5'-		-3'
	Sequence Required
OD / µg / nmol

	OD/µg Required Must be greater than 0

Results

Sequence:

Complement:

Protein:

nmoles:

µg:

OD₂₆₀:

nmole/OD₂₆₀:

µg/OD₂₆₀:

Anhydrous MW:

Tm (Basic):

Tm (Salt Adjusted):

GC Content:

Extinction Coefficient:

Length:

Assumptions:
Calculation assumes that the annealing occurs under the standard conditions of 50 nM primer and pH 7.0.
Salt adjusted Tm at 50 mM Na+