Oligonucleotide-Peptide Bioconjugations
Oligonucleotide-peptide conjugates have a wide application in molecular biology, as it gives an opportunity to selectively effect gene expression by using peptide translocation signals and direct oligonucleotide to the cellular compartment of choice.
Combining these two powerful peptide and oligonucleotide synthesis expertise and cross linking and bioconjugation chemistries, we routinely assist client in creating bi-functional or tri-functional chimeric biopolymer such as peptide-oligonucleotide. As always, quality is guaranteed!
Services we offered:
PNA-oligonucleotide conjugates
Single amino acid to oligo conjugation
Peptide to oligonucleotide without Cs
DNA-peptide-RNA conjugations and more...
Advantages
Use cellular translocationsignals
Enable targeted transport of nucleic acids to cellular compartments
Facilitate transport of nucleic acids through cell membranes
Applications
Delivery of antisense and siRNA for gene silencing experiments
Targeted direction of nucleic acids into cells
Standard Service Includes
| Requirements |
Peptide |
Oligonucleotide |
| Length |
5-15 amino acids |
8-30 DNA, RNA, PNA bases |
| Purity |
>85-95% |
HPLC purified |
| Coupling via |
N- or C-terminal Cys |
3'- or 5'-terminal amino modified |
| Restrictions |
only one Cys per peptide |
none |
| Modifications |
none |
| Other possible combinations: |
| • |
PTO or LNA bases |
| • |
Additional dye modification |
| (such as Fluorescein or CY3) |
|
Quantity
| Conjugate yield in OD |
1 ODU |
2 ODU |
3 ODU |
5 ODU |
| Optical density at λ= 260 nm |
Price: Standard price covers labor and materials including the cost of activated oligo preparation, peptide conjugation procedure, purification and MS analysis. For details,
contact us.