Chemical Phosphorylation
   
  Oligonucleotides containing a 5’-phosphate group have various applications, e.g. as linkers and adapters, in cloning and gene construction, and in the ligase chain reaction. This chemical phosphorylation provide a cost-effective alternative to enzymatic methods by direct introducing 5'-PO4 or 3'-PO4 group during synthesis. this phosphorylation permits the labeling of large quantities and is more reproducible than enzymatic procedure.
   
5'-phosphorylation

 

3' phosphorylation
           
  Abbreviation: /5Phos/   Abbreviation: /3Phos/
  MW: 80   MW: 80