Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1 (3). Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog (4). In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles (5). It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death (6).
Additional Names: APG7 (NT), Autophagy protein 7 / ATG7
Left: Western blot analysis of APG7 in L1210 cell lysate with APG7 antibody at (A) 1, (B) 2 and (C) 4 µg/ml.
Below: Immunocytochemistry of APG7 in L1210 cells with APG7 antibody at 10 µg/ml.
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APG7 antibody was raised against a 17 amino acid peptide from near the amino terminus of human APG7.
Purification: Affinity chromatography purified via peptide column
Clonality and Clone: This is a polyclonal antibody.
Host: APG7 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen: Human APG7 / Autophagy protein 7 / ATG7 (N-Terminus) Peptide (Cat. No. 3617P)
Application: APG7 antibody can be used for the detection of APG7 by Western blot at 1 - 2 µg/ml.(Optimal dilution should be determined by user). L1210 cell lysate can be used as positive control. APG7 antibody is human and mouse reactive.
Tested Application(s): E, WB, ICC
Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide:Cat. No. 3617P - APG7 Peptide
Long-Term Storage: APG7 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cat. No. 1284 - L1210 Cell Lysate
Accession Number: NP_006386
Short Description: (NT) An autophagy related protein
- Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.
- Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
- Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13.
- Mizushima N, Noda T, Yoshimori T, et al. A protein conjugation system essential for autophagy. Nature 1998; 395:395-8.