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Home » Antibody and Immunochemistry » Polyclonal Antibody Services » Select By Host Animals » Rabbit Polyclonal Antibody
custom rabbit polyclonal antibody, guarantee ELSIA results

Custom Rabbit Polyclonal Antibody

Bio-Synthesis (BSI) provides rabbit polyclonal antibody production in its USDA-licensed facility using pathogen-free, barrier-raised New Zealand White Rabbits. These barrier-raised rabbits often produce antibodies with higher titers and provide greater reproducibility more often or between animals more often than conventional animals. We can produce polyclonal antibodies with a wide variety of antigens, from bacteria, viruses, fungi, fusion proteins, synthetic peptides, and plasmid DNA, as well as a variety of other proteins.

Typical antigen requirement is 2 mg conjugated peptide/animal and 1 mg protein antigens/animal. However, amounts may vary due to antigen availability and additional antigen is required for affinity purification.

Three production schedules are provided for our rabbit antibody services. We can produce polyclonal antibodies from a few to several hundred rabbits per run, using our standard protocol or by a customer-designed protocol. We also supply polyclonal production from other species, including goat, sheep, chicken, rat, mouse, and guinea pig. Additional animals are available for all service packages.

BSI supplies a Polyclonal Antibody Information Sheet with each inquiry to assist in meeting our customer specifications.

Rabbit Polyclonal Antibody Service Packages

All of our rabbit polyclonal antibody packages can be prolonged with an additional immunization every 28 days which includes two additional serum after the immunization. Production extension is an additional charge of animal maintenance fee.

Request a quote or contact us for your Rabbit Polyclonal Antibody packages.

Service Descriptions Antigen Packages   Purification
 
Peptide
User
Supplied
Conjugation Affinity Protein A
EE-PE-RA070 Economy Express: peptide >70%, conjugation, 56-70 days. √   √    
ES-PE-RA090 Economy Standard: peptide >70%, conjugation, 70-90 days. √   √    
ES-PE-RA090A Economy Class Plus Protein A Column Purified: peptide >70%, conjugation, 90-120 days, protein A purification √   √   √
FS-PE-RA090 First Class Standard: peptide >80%, conjugation, 70-90 days. √   √    
FP-PE-RA120A First Class Plus Protein A Column Purified: peptide >80%, conjugation, 90-120 days, protein A purification. √   √   √
FP-PE-RA120I First Class Plus Immunoaffinity Purified: peptide >80%, conjugation, 90-120 days, peptide affinity √   √ √  
SS-PE-RA090 Super Class Standard: peptide >90%, conjugation, 70-90 days √   √    
SP-PE-RA120A Super Class Plus Protein A Column Purified: peptide >90%, conjugation, 90-120 days, protein A purification √   √   √
SP-PE-RA120I Super Class Plus Immunoaffinity Purified: peptide >90%, conjugation, 90-120 days, peptide affinity purification √   √ √  
SP-PE-RA120M Super Class Plus Western Blot: peptide >90%, conjugation, 90-120 days, affinity purification, western analysis √   √ √  
US-PR-RA090 User-Supplied Protein Antibody Package, 70-90 days   √      
US-PR-RA90A User-Supplied Protein Plus Protein A Column Purified Antibody Package, 70-90 days   √     √
US-PE-RA120I User-Supplied Peptide Plus Immunoaffinity Purified Antibody Package, 90-120 days   √ Optional √  
US-PR-RA120I User-Supplied Protein Plus Immunoaffinity Purified Antibody Package, 90-120 days   √   √  
Three production schedules are available for each Rabbit Polyclonal Antibody package along with its descriptions.

Available Production Time Frame:
  • 56-70 days
  • 70-90 days
  • 90-120 days
Custom Peptide Rabbit Polyclonal Antibody Services
Economy Express Peptide Antibody Package (Rabbit)
Package: EE-PE-RA070 (56 Days Immunization Protocol) Time Frame 56-70 days and delivery
Peptide Synthesis > 70% (<20 a.a. 15 mg) ~8 ml control serum
KLH/BSA conjugation via Cysteine added on C- or N-term of Peptide up to 100 ml crude antiserum
2 rabbits, 2 control serum (1/animal), 2 initial inoculations (1/animal), 6 boosters (3/animal), 2 test serum (1/animal), 4 production serum (2/animal) 5 mg of unconjugated peptide
ELISA titer assay on collection serum MS, HPLC of peptide, ELISA reports
  See immunization and delivery schedule.
 
Economy Standard Peptide Antibody Package (Rabbit)
Package: ES-PE-RA090 (70 Days Immunization Protocol) Time Frame 70-90 days and delivery
Peptide Synthesis > 70% (<20 a.a. 15 mg) ~8 ml control serum
KLH/BSA conjugation via Cysteine added on C- or N-term of Peptide up to 150 ml crude antiserum
2 rabbits, 2 control serum (1/animal), 2 initial inoculations (1/animal), 8 boosters (4/animal), 6 production serum (3/animal) 5 mg of unconjugated peptide
ELISA titer assay on collection serum MS, HPLC of peptide, ELISA reports
  See immunization and delivery schedule.
 
First Class Standard Peptide Antibody Packages (Rabbit)
First Class Standard Peptide Antibody Package (Rabbit)
Package: FS-PE-RA090 (70 Days Immunization Protocol) Time Frame 70-90 days and delivery
Peptide Synthesis > 80% (<20 a.a. 15 mg) ~8 ml control serum
KLH/BSA conjugation via Cysteine added on C- or N-term of Peptide up to 150 ml crude antiserum
2 rabbits, 2 control serum (1/animal), 2 initial innoculatiions (1/animal), 8 boosters (4/animal), 6 production serum (3/animal) 5 mg of unconjugated peptide
ELISA titer assay on collection serum MS, HPLC of peptide, ELISA reports
  See immunization and delivery schedule.
 
First Class Plus Protein A Column Purified Peptide Antibody (Rabbit)
Package: FP-PE-RA120A (84 Days Immunization Protocol) Time Frame 90-120 days and delivery
Peptide Synthesis > 80% (<20 a.a. 15 mg) ~8 ml control serum
KLH/BSA conjugation via Cysteine added on C- or N-term of peptide up to 250 mgs protein A purified antibodies from 25 ml antiserum
2 rabbits, 2 control serum (1/animal), 2 initial inoculations (1/animal), 10 boosters (5/animal), 2 test serum (1/animal), 6 production serum (3/animal) Remaining unpurified antiserum
Protein A purification 5 mg of unconjugated peptide, MS, HPLC and ELISA reports
ELISA titer assay on collection serum and protein A purified samples Electronic image of SDS analysis
SDS PAGE analysis See immunization and delivery schedule.
 
First Class Plus Immunoaffinity Purified Peptide Antibody Package (Rabbit)
Package: FP-PE-RA120I (84 Days Immunization Protocol) Time Frame 90-120 days and delivery
Peptide Synthesis > 80% (<20 a.a. 20 mg) ~8 ml control serum
KLH/BSA conjugation via Cysteine added on C- or N-term of Peptide up to 25 mgs of peptide affinity purified antibodies from 25 ml antiserum
2 rabbits, 2 control serum (1/animal), 2 initial inoculations (1/animal), 10 boosters (5/animal), 2 test serum (1/animal), 6 production serum (3/animal) Remaining unpurified antiserum
Peptide immobilization on sepharose beads ELISA titer assay on collection serum
Loading of the antiserum on the affinity column and elution of bound antibodies 5 mg of unconjugated peptide, MS, HPLC of peptide and ELISA reports
ELISA titer assay on crude collection serum and peptide immunopurified antibodies Electronic image of SDS analysis
SDS PAGE analysis See immunization and delivery schedule.
 
Super Class Packages
Super Class Standard Peptide Antibody Package (Rabbit)
Package: SS-PE-RA090 (70 Days Immunization Protocol) Time Frame 70-90 days and delivery
Peptide Synthesis > 90% (<20 a.a. 15 mg) ~8 ml control serum
KLH/BSA conjugation via Cysteine added on C- or N-term of Peptide up to 150 ml crude antiserum
2 rabbits, 2 control serum (1/animal), 2 initial inoculations (1/animal), 8 boosters (4/animal), 6 production serum (3/animal) 5 mg of unconjugated peptide
ELISA titer assay on collection serum MS, HPLC of peptide, ELISA reports
  See immunization and delivery schedule..
 
Super Class Plus Protein A Column Purified Peptide Antibody (Rabbit)
Package: SP-PE-RA120A (84 Days Immunization Protocol) Time Frame 90-120 days and delivery
Peptide Synthesis > 90% (<20 a.a. 15 mg) ~8 ml control serumm
KLH/BSA conjugation via Cysteine added on C- or N-term of Peptide up to 250 mgs of protein A purified antibodies from 25 ml antiserum
2 rabbits, 2 control serum (1/animal), 2 initial inoculations (1/animal), 10 boosters (5/animal), 2 test serum (1/animal), 6 production serum (3/animal) Remaining unpurified antiserum
Protein A purification 5 mg of unconjugated peptide, MS, HPLC and ELISA reports
ELISA titer assay on collection serum and protein A purified samples Electronic image of SDS analysis
SDS PAGE analysis See immunization and delivery schedule.
 
Super Class Plus Immunoaffinity Purified Peptide Antibody Package (Rabbit)
Package: SP-PE-RA120I (84 Days Immunization Protocol) Time Frame 90-120 days and delivery
Peptide Synthesis >90% (<20 a.a. 20 mg) ~8 ml control serumm
KLH/BSA conjugation via Cysteine added on C- or N-term of Peptide up to 25 mg of peptide affinity purified antibodies from 25 ml antiserum
2 rabbits, 2 control serum (1/animal), 2 initial inoculations (1/animal), 10 boosters (5/animal), 2 test serum (1/animal), 6 production serum (3/animal) Remaining unpurified antiserum
Peptide immobilization on sepharose beads 5 mg of unconjugated peptide
Loading of the antiserum on the affinity column and elution of bound antibodies MS, HPLC of peptide, ELISA reports
ELISA titer assay on crude collection serum and peptide immunopurified antibodies Electronic image of SDS analysis
SDS PAGE analysis See immunization and delivery schedule.
 
Super Class Plus M Immunoaffinity Purified Peptide Antibody Package (Rabbit) - Western Blot Characterized
Package: SP-PE-RA120M (84 Days Immunization Protocol) Time Frame 90-120 days and delivery
Peptide Synthesis > 90% (<20 a.a. 20 mg) ~8 ml control serum
KLH/BSA conjugation via Cysteine added on C- or N-term of Peptide up to 25 mg of peptide affinity purified antibodies from 25 ml antiserum
2 rabbits, 2 control serum (1/animal), 2 initial inoculations (1/animal), 10 boosters (5/animal), 2 test serum (1/animal), 6 production serum (3/animal) Remaining unpurified antiserum
Peptide immobilization on sepharose beads Peptide affinity purified column
Loading of the antiserum on the affinity column and elution of bound antibodies 5 mg of unconjugated peptide
ELISA titer assay on crude collection serum and peptide immunopurified antibodies MS, HPLC of peptide, ELISA reports
Western Blot analysis on purified antibodies Electronic image of the blot is provided
4 lysate panels See immunization and delivery schedule.
 
User-Supplied Immunogen - Peptide/Recombinant and Fusion Protein
User-Supplied Protein Antibody Package (Rabbit)
Package: US-PR-RA090 (70 Days Immunization Protocol) Time Frame 70-90 days and delivery
Conjugation to carrier (optional, additional fee) ~8 ml control serum
2 rabbits, 2 control serum (1/animal), 2 initial inoculations (1/animal), 8 boosters (4/animal), 6 production serum (3/animal) up to 150 ml crude antiserum
ELISA titer assay of collection serum ELISA report
  See immunization and delivery schedule.
Antigen requirement: 1.5-2.0 mgs of protein. The concentration should be 1 mg/ml is a good target range, minimum >0.5 mg/ml in PBS buffered solution pH 7.4.
See details on antigen preparation
 
User-Supplied Protein Plus Protein A Column Purified Antibody Package (Rabbit)
Package: US-PR-RA90A (70 Days Immunization Protocol) Time Frame 70-90 days and delivery
Conjugation to carrier (optional, additional fee) ~8 ml control serum
2 rabbits, 2 control serum (1/animal), 2 initial inoculations (1/animal), 8 boosters (4/animal), 6 production serum (3/animal) up to 250 mgs protein A purified antibodies from 25 ml antiserum
ELISA titer assay of collection serum and protein A purified sample ELISA report
SDS-PAGE analysis Electronic image of SDS analysis
  See immunization and delivery schedule.
Antigen requirement: 1.5-2.0 mgs of protein. The concentration should be 1 mg/ml is a good target range, minimum >0.5 mg/ml in PBS buffered solution pH 7.4.
See details on antigen preparation
 
User-Supplied Peptide Immunoaffinity Purified Antibody Package (Rabbit)
Package: US-PE-RA120I (84 Days Immunization Protocol) Time Frame 90-120 days and delivery
Conjugation to Carrier Protein (optional, additional fee) ~8 ml control serum
2 rabbits, 2 control serum (1/animal), 2 initial inoculations (1/animal), 10 boosters (5/animal), 2 test serum (1/animal), 6 production serum (3/animal) User supplied-peptide affinity purified antibodies from 25 ml antiserum
Peptide immobilization on sepharose beads Peptide-bounded affinity column
Loading of the antiserum on the affinity column and elution of bound antibodies Remaining unpurified antiserum
ELISA titer assay on crude collection serum and peptide immunopurified antibodies ELISA report
SDS-PAGE analysis Electronic image of SDS analysis
  See immunization and delivery schedule.
Antigen requirement:  4.5-6.0 mgs of peptide conjugates for immunization, 5-10 mgs of unconjugated peptide for purification. The concentration of peptide conjugates should be >1 mg/ml in PBS buffered solution pH 7.4.
See details on antigen preparation
 
User-Supplied Protein Immunoaffinity Purified Antibody Package (Rabbit)
Package: US-PR-RA120I (84 Days Immunization Protocol) Time Frame 90-120 days and delivery
Protein analysis by SDS-PAGE ~8 ml control serum
2 rabbits, 2 control serum (1/animal), 2 initial inoculations (1/animal), 10 boosters (5/animal), 2 test bleeds (1/animal), 6 production bleeds (3/animal) User supplied-protein affinity purified antibodies from 25 ml antiserum
Protein conjugated to affinity resin (protein dialysis in PBS) Protein-bounded affinity column
Loading of the antiserum on the affinity column and elution of bound antibodies Remaining unpurified antiserum
ELISA titer assay of crude collection serum and protein immunopurified antibodies ELISA report
SDS-PAGE analysis Electronic image of SDS analysis
  See immunization and delivery schedule.
Antigen requirement: 8-10 mgs of protein (2-3 mgs for immunization, 5 mgs for purification) The concentration should be 1 mg/ml is a good target range, minimum >0.5 mg/ml in PBS buffered solution pH 7.4.
See details on antigen preparation

Rabbit Polyclonal Antibody Production Protocols

Develop your own customized host species and immunization schedule and Bio-Synthesis will make it for you.

We offer two standard custom antibody protocols (combinations of hosts and protocol time frame) From peptide design, synthesis, and conjugation, to antibody generation and purification, Bio-Synthesis offers a complete package to meet your protein detection needs. User-design production protocol also available and can also be modified from existing package.
Serum Production Specification:
Immunization schedule listed below are approximate (± 2 days).

  • Two New Zealand white rabbits will be utilized for each MAP-peptide, conjugated-peptide immunogen, or protein immunogen packages
  • Total material required for injection for the program below is 650-900 ug protein/animal, 2 mg conjugated peptide/animal. If we are conjugating your protein we assume a 20% loss of protein during conjugation. Total amount of antiserum expected from a rabbit on the program below is approximately ~50-75 mL/animal.
  • Immunogen is emulsified in Freund's Complete Adjuvant (FCA) for initial injections. Freund's Incomplete Adjuvant (FIA) is used for all subsequent injections (boosts).
  • Continuation of project past our standard immunization schedule is offered on a month to month basis. The standard extension protocol includes two boosters, one serum collection, and maintenance for one month. Terminal bleeds may be obtained at any time yielding approximately ~75 ml/animal of additional serum taken upon customer request.
 
Program Development of Rabbit Polyclonal Antibody: 56-70 Dayss
Procedure Protocol Days* Description
Phase I - Antigen design, synthesis and preparation
Antigen Preparation ~ 5 - 10 days Peptide synthesis and purification (if required) and antigen conjugation
Phase II - Immunization Schedule
Control serum collection 
Initial Inoculation		 Day 0
NZQ female rabbits and Control serum (~5 ml serum/rabbit)
Primary Immunization with 500 µg peptide or 250 ug protein per host with FCA in 10 SQ sites
1st Booster Day 14 Boost with 500 µg peptide or 125 µg protein per host with FIA in 4 SQ sites
2nd Booster Day 28 Week 4 Test serum ~5 ml/rabbit
Boost with 500 µg peptide or 125 µg protein per host with FIA in 4 SQ sites
1st Serum Collection

3rd Booster 		Day 42 Week 6 Colleton serum ~25 ml/rabbit
Boost with 500 µg peptide or 125 µg protein per host with FIA in 4 SQ sites
2nd Serum Collection

ELISA 		Day 56-62 Week 8 Colleton serum ~25 ml/rabbit

ELISA assay of control and week 8 collection serum
Final Shipment

Project Completion Verification 		Day 63-64 Shipping of control, week 6 and 8 collection serum

Verify disposition of rabbits, extension or termination
Instruction Due Date Day 70 Termination of animals if instruction not received
 
Program Development of Rabbit Polyclonal Antibody: 70-90 Days
Procedure Protocol Days* Description
Phase I - Antigen design, synthesis and preparation
Antigen Preparation ~ 5 - 10 days Peptide synthesis and purification (if required) and antigen conjugation
Phase II - Immunization Schedule
Control serum collection
Initial Inoculation
Day 0
NZQ female rabbits and Control serum (~5 ml serum/rabbit)
Primary Immunization with 500 µg peptide or 250 ug protein per host with FCA in 10 SQ sites
1st Booster Day 14 Boost with 500 µg peptide or 125 µg protein per host with FIA in 4 SQ sites
2nd Booster Day 28 Boost with 250 µg peptide or 125 µg protein per host with FIA in 4 SQ sites
1st Serum Collection

3rd Booster 		Day 42 Week 6 Collection serum ~25 ml/rabbit
Boost with 250 µg peptide or 125 µg protein per host with FIA in 4 SQ sites
2nd Serum Collection

4th Boosterr

ELISA 		Day 56-63 Week 8 Collection serum ~25 ml/rabbit

Boost with 250 µg peptide or 125 µg protein per host with FIA in SQ 4 sitess

ELISA assay of control and week 8 collection serum
1st Shipment

Project Completion Verification 		Day 63-64 Shipping of control, week 6 and 8 collection serum

Verify disposition of rabbits, extension or termination
3rd Serum Collection Day 70 Week 10 Collection serum ~25 ml/rabbit
Final Shipment Day 75 Shipping of week 10 collection serum
Instruction Due Date Day 84 Termination of animals if instruction not received
 
Program Development of Rabbit Polyclonal Antibody: 90-120 Days
Procedure Protocol Days* Description
Phase I - Antigen design, synthesis and preparation
Antigen Preparation ~ 5 - 10 days Peptide synthesis and purification (if required) and antigen conjugation
Phase II - Immunization Schedule
Control serum collection
Initial Inoculation
Day 0
NZQ female rabbits and Control serum (~5 ml serum/rabbit)
Initial Inoculation with 500 µg peptide or 250 µg protein per host with FCA in SQ 4 sites
1st Booster Day 14 Boost with 500 µg peptide or 125 µg protein per host with FIA in 4 SQ sites
2nd Booster Day 28 Boost with 250 µg peptide or 125 µg protein per host with FIA in 4 SQ sites
Test serum

3rd Booster 		Day 42 Week 6 Test serum ~5 ml/rabbit

Boost with 250 µg peptide or 125 µg protein per host with FIA in SQ 4 sites
1st Serum Collection

4th Booster 		Day 56 Week 8 Collection serum ~25 ml/rabbit

Boost with 250 µg peptide or 125 µg protein per host with FIA in 4 SQ sites
2nd Serum Collection

5th Booster

ELISA Titer Assay 		Day 70-75 Week 10 Collection serum ~25 ml/rabbit

Boost with 250 µg peptide or 125 µg protein per host with FIA in 4 SQ sites

ELISA assay of control, week 6 and 10 collection serum
1st Shipment

Project Completion Verification 		Day 77 Shipping of control, 6, 8 and 10 collection serum and ELISA report

Verify disposition of rabbits, extension or termination
3rd Serum Collection Day 84 Week 12 Collection serum ~25 ml/rabbit
Phase III - post production sample processingg

Instruction Due Date 		Day 85-98 Preparation of phase III sample processingg

Termination of animals if instruction for extension or termination not received
Phase III - Post Production Sample Processing
Serum Processing 2 weeks affinity column preparation, purification and/or labeling
ELISA and/or SDS Analysis 1 week Analysis of purified sample
Final Shipment Day 108 Shipping of purified sample, unpurified week 12 serum and documents
 
Links and Resourcess

Forms:
Order/Quote Page

Additional Phospho-Specific Antibody Information:
Phospho-Specific Life-Cycle Illustration

Sci Apps:
Peptide Property Calculator
Peptide Design Library
Oligo Designer

Useful Links
Reference/Citing:
1.
Different prelamin A forms accumulate in human fibroblasts: a study in experimental models and progeria
2.
1,25-DIHYDROXYVITAMIN D3-INDUCED GENES IN OSTEOBLASTS: UNCOVERING NEW FUNCTIONS FOR MENINGIOMA 1 AND SEMAPHORIN 3B IN SKELETAL PHYSIOLOGY
3.
Fetal Death in Mice Lacking 5α-Reductase Type 1 Caused by Estrogen Excess
4.
A splicing variant of a death domain protein that is regulated by a mitogen-activated kinase is a substrate for c-Jun N-terminal kinase in the human central nervous system
Ordering Services
To place an order or request a quotation, please proceed to our custom Order/Quote page or Contact Us for any other questions.
Order can be placed with either a PO (Purchase Order) or credit card. You may place order online using our secure online ordering system. Our customer service representatives are available 24 hours a day, Monday through Friday, to assist you.
   
We accept POs and major credit cards ( ). Your credit card will be billed under "Bio-Synthesis, Inc." Please complete and return our Business Credit Application. For international orders, we must apply the full charge at the time of the order is placed. In the unlikely event that any given order cannot be filled, our guarantee will take the form of a full refund.
 
 
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