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Custom Rat Polyclonal Antibody Services

Bio-Synthesis (BSI) provides polyclonal antibody production in its USDA-licensed facility using pathogen-free, VAF Long-Evans Female rats. We can produce polyclonal antibodies with a wide variety of antigens, from bacteria, viruses, fungi, fusion proteins, synthetic peptides, and plasmid DNA, as well as a variety of other proteins.

Typical antigen requirement is 1.5 mg conjugated peptide/animal, and 800 µg protein antigen/animal. However, amounts may vary due to antigen availability and additional antigen is required for affinity purification.

Two production schedules are provided for our rat antibody services. We can produce polyclonal antibodies in a few to several hundred rats per run, using our standard protocol or by a customer-designed protocol. We also supply polyclonal production from other species, including goat, sheep, chicken, mouse, rabbit, and guinea pig. Additional animals are available for all service packages.

BSI supplies a Polyclonal Antibody Information Sheet with each inquiry to assist in meeting our customer specifications.

Rat Polyclonal Antibody Packages

Don't see what you need? Just ask - in most cases we can accommodate your request. If you require help in choosing the services for your polyclonal antibody production, feel free to consult our experts.

All of our antibody packages can be prolonged with an additional immunization every 28 days which includes two additional serum after the immunization. Production extension is an additional charge of animal maintenance fee.

Request a quote or contact us for your Rat Polyclonal Antibody packages.
Service Descriptions Antigen Packages   Purification
 
Peptide
User
Supplied
Conjugation Affinity G
SS-PE-RT090 Super Class Standard (Rat) Antibody Package: peptide synthesis >90%, conjugation, 70-90 days √   √    
SP-PE-RT120G Super Class Plus G (Rat) Antibody Package: peptide synthesis >90%, conjugation, protein A purification, 90-120 days √   √   √
US-PR-RT090 User-Supplied Protein (Rat) Antibody Package: 70-90 days   √      
US-PR-RT120G User-Supplied Protein G (Rat) Antibody Package: protein G column purification, 90-120 days   √     √
Two production schedules are available for each Rat Polyclonal Antibody package along with its descriptions.

Available Production Time Frame:
  • 70-90 days
  • 90-120 days
Custom Peptide Polyclonal Antibody Services
Super Class Standard Peptide Antibody Package (Rat)
Package: SS-PE-RT090 (70 Days Immunization Protocol) Time Frame 70-90 days and delivery
Peptide Synthesis > 90% (<20 a.a. 15 mg) ~100 ul control serum from each rat
KLH/BSA conjugation via Cysteine added on C- or N-term of peptide ~5 ml of antiserum from all rats
3 rats, 3 control serum (1/animal), 3 initial inoculations (1/animals), 12 boosters (4/animal), 9 collection serum (3/animal) 5 mg of unconjugated peptide, MS, HPLC and ELISA reports
ELISA titer assay on collection serum See immunization and delivery schedule.
 
Super Class Plus Protein G Column Purified Peptide Antibody Package (Rat)
Package: SP-PE-RT120G (84 Days Immunization Protocol) Time Frame 90-120 days and delivery
Peptide Synthesis > 90% (<20 a.a. 15 mg) ~100 ul control serum from each rat
KLH/BSA conjugation via Cysteine added on C- or N-term of peptide Protein G affnity purified antibody from ~5 ml
3 rats, 3 control serum (1/animal), 3 initial inoculations (1/animals), 15 boosters (5/animal), 12 collection serum (4/animal) 5 mg of unconjugated peptide
Protein G purification MS, HPLC and ELISA reports
ELISA titer assay on collection serum and protein G purified samples Electronic image of SDS analysis
SDS PAGE analysis See immunization and delivery schedule.
 
User Supplied Immunogen - Peptide/Recombinant and Fusion Protein
User-Supplied Protein Antibody Package (Rat)
Package: US-PR-RT090 (70 Days Immunization Protocol) Time Frame70-90 days and delivery
Conjugation to carrier (optional, additional fee) ~100 ul control serum from each rat
3 rats, 3 control serum (1/animal), 3 initial inoculations (1/animals), 12 boosters (4/animal), 9 collection serum (3/animal) ~5 ml of antiserum from all rats
ELISA titer assay of collection serum ELISA report
  See immunization and delivery schedule.
Antigen requirement: 2.0-2.5 mgs of protein. The concentration should be 1 mg/ml is a good target range, minimum >0.5 mg/ml in PBS buffered solution pH 7.4.
See details on antigen preparation
 
User-Supplied Protein G Purified Antibody Package (Rat)
Package: US-PR-RT120G (84 Days Immunization Protocol) Time Frame 90-120 days and delivery
Conjugation to carrier (optional, additional fee) ~100 ul control serum from each rat
3 rats, 3 control serum (1/animal), 3 initial inoculations (1/animals), 15 boosters (5/animal), 12 collection serum (4/animal) Protein G affnity purified antibody from ~5 ml
Protein G purification ELISA report
ELISA titer assay of collection serum and protein G purified samples Electronic image of SDS analysis
SDS-PAGE analysis See immunization and delivery schedule.
Antigen requirement: 2.5-3.0 mgs of protein. The concentration should be 1 mg/ml is a good target range, minimum >0.5 mg/ml in PBS buffered solution pH 7.4.
See details on antigen preparation
We offer two standard custom antibody protocols (combinations of hosts and protocol time frame) From peptide design, synthesis, and conjugation, to antibody generation and purification, Bio-Synthesis offers a complete package to meet your protein detection needs. User-design production protocol also available and can also be modified from existing package.
Serum Production Specification:
Immunization schedule listed below are approximate (± 2 days).

  • Three rats will be utilized for each MAP-peptide, conjugated - peptide immunogen, or protein immunogen packages
  • Total material required for injection for the program below is 650-800 ug protein/animal, 1.3-1.6 mg peptide/animal conjugate. If we are conjugating your protein we assume a 20% loss of protein during conjugation. Total amount of antiserum expected from a rat on the program below is approximately ~1.5 mL/animal.
  • Immunogen is emulsified in Freund's Complete Adjuvant (FCA) for initial injections. Freund's Incomplete Adjuvant (FIA) is used for all subsequent injections (boosts).
  • Continuation of project past our standard immunization schedule is offered on a month to month basis. The standard extension protocol includes two boosters, one serum collection, and maintenance for one month. Terminal bleeds may be obtained at any time yielding approximately ~6 ml/animal of additional serum taken upon customer request.
 
Program Development of Rat Polyclonal Antibody: 70-90 Days
Procedure Protocol Days* Description
Phase I - Antigen design, synthesis and preparation
Antigen Preparation ~ 10 - 15 days Peptide synthesis and purification (if required) and antigen conjugation
Phase II - Immunization Schedule
Control serum collection

Initial Inoculation
Day 0
3 VAF Long-Evans Female and Control serum (~0.1 ml serum/rat)
Initial Inoculation with 250 µg peptide or 200 µg protein per host with FCA in SQ 4 sites

 

1st Booster Day 14 Boost with 250 µg peptide or 100 µg protein per host with FIA in SQ 4 sites
2nd Booster Day 28 Boost with 250 µg peptide or 100 µg protein per host with FIA in SQ 4 sites
1st Serum Collection

3rd Booster 		Day 42 Week 6 Collection serum ~0.5 ml/rat

Boost with 250 µg peptide or 100 µg protein per host with FIA in SQ 4 sites
2nd Serum Collection


4th Booster 		Day 56 Week 8 Collection serum ~0.5 ml/rat

Boost with 250 µg peptide or 100 µg protein per host with FIA in SQ 4 sites
ELISA Titer Assay

1st Shipment

Project Completion Verification 		Day 60 ELISA assay of control, week 8 collection serum

Shipping of control, week 6 and 8 collection serum

Verify disposition of rats, extension or termination
3rd Serum Collection Day 70 Week 10 Collection serum ~0.5 ml/rat
Final Shipment Day 75 Shipping of week 10 collection serum
Instruction Due Date Day 84 Termination of animals if instruction not received
 
Program Development of Rat Polyclonal Antibody: 90-120 Days
Procedure Protocol Days* Description
Phase I - Antigen design, synthesis and preparation
Antigen Preparation ~ 10 - 15 days Peptide synthesis and purification (if required) and antigen conjugation
Phase II - Immunization Schedule
Control serum collection


Initial Inoculation
Day 0
3 VAF Long-Evans Female and Control serum (~0.1 ml serum/rat)
Initial Inoculation with 250 µg peptide or 200 µg protein per host with FCA in SQ 4 sites
1st Booster Day 14 Boost with 250 µg peptide or 100 µg protein per host with FIA in SQ 4 sites
2nd Booster Day 28 Boost with 250 µg peptide or 100 µg protein per host with FIA in SQ 4 sites
1st Serum Collection

3rd Booster 		Day 42 Week 6 Collection serum ~0.5 ml/rat

Boost with 250 µg peptide or 100 µg protein per host with FIA in SQ 4 sites
2nd Serum Collection

4th Booster 		Day 56 Week 8 Collection serum ~0.5 ml/rat

Boost with 250 µg peptide or 100 µg protein per host with FIA in SQ 4 sites
3rd Serum Collection

5th Booster

ELISA Titer Assay 		Day 70-75 Week 10 Collection serum ~0.5 ml/rat

Boost with 250 µg peptide or 100 µg protein per host with FIA in SQ 4 sites

ELISA assay on control, week 6, 8, and 10 collection serum
1st Shipment

Project Completion Verification 		Day 77 Shipping of control, 6, 10 collection serum and ELISA report

Verify disposition of rats, extension or termination
4th Serum Collection Day 84 Week 12 Collection serum ~0.5 ml/rat
Phase III - post production sample processing

Instruction Due Date 		Day 98 Preparation of phase III sample processing

Termination of animals if instruction for extension or termination not received
Phase III - Post Production Sample Processing
Serum Processing ~ 2 weeks affinity column preparation, purification and/or labeling
ELISA and/or SDS Analysis ~ 1 weeks Analysis of purified sample
Final Shipment Day 108 Shipping of purified sample, unpurified week 12 serum and documents
Links and Resources

Forms:
Order/Quote Page

Additional Phospho-Specific Antibody Information:
Phospho-Specific Life-Cycle Illustration

Sci Apps:
Peptide Property Calculator
Peptide Design Library
Oligo Designer

Useful Links
Reference/Citing:
1.
Different prelamin A forms accumulate in human fibroblasts: a study in experimental models and progeria
2.
1,25-DIHYDROXYVITAMIN D3-INDUCED GENES IN OSTEOBLASTS: UNCOVERING NEW FUNCTIONS FOR MENINGIOMA 1 AND SEMAPHORIN 3B IN SKELETAL PHYSIOLOGY
3.
Fetal Death in Mice Lacking 5α-Reductase Type 1 Caused by Estrogen Excess
4.
A splicing variant of a death domain protein that is regulated by a mitogen-activated kinase is a substrate for c-Jun N-terminal kinase in the human central nervous system
Ordering Services
To place an order or request a quotation, please proceed to our custom Order/Quote page or Contact Us for any other questions.
Order can be placed with either a PO (Purchase Order) or credit card. You may place order online using our secure online ordering system. Our customer service representatives are available 24 hours a day, Monday through Friday, to assist you.
   
We accept POs and major credit cards ( ). Your credit card will be billed under "Bio-Synthesis, Inc." Please complete and return our Business Credit Application. For international orders, we must apply the full charge at the time of the order is placed. In the unlikely event that any given order cannot be filled, our guarantee will take the form of a full refund.
 
 
 
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