Custom fluorescent dye-labeled DNA and RNA oligos for qPCR, FISH, smFISH, smFRET, imaging, flow cytometry and multiplex assay workflows.
Bio-Synthesis supports custom fluorescent dye-labeled oligonucleotide synthesis for research, diagnostic development and advanced molecular biology workflows that require precise fluorescence detection.
Fluorescent dye selection should be matched to the instrument channel, target application, quencher compatibility, spectral overlap, probe position, linker spacing and downstream purification strategy.
Supported formats include 5′ dye-labeled oligos, 3′ dye-labeled oligos, internally labeled probes, dual-labeled hydrolysis probes, FRET probes, FISH/smFISH probes, near-infrared oligo probes and custom fluorescent oligo panels.
Visible, organized dye-family catalog for common fluorescent oligo labeling options. Values are practical reference points; final dye choice should be matched to your instrument filter set and assay conditions.
Use the color band to quickly view fluorescent dye options by emission color.
Blue dye options
Cyan dye options
Green dye options
Yellow-Green dye options
Yellow dye options
Orange dye options
Orange-Red dye options
Red dye options
Far-Red dye options
NIR dye options
IR/NIR dye options
Choose a wavelength band to find dyes by emission range. This is useful when selecting fluorophores for lasers, filters, qPCR channels, microscopy or multiplex panels.
Fluorescent probes perform best when the dye is selected together with probe architecture, assay type, channel spacing and QC plan.
Match excitation/emission to your instrument filters and avoid overlapping reporters in multiplex panels.
Choose 5′, 3′ or internal placement based on whether the dye acts as a reporter, FRET donor/acceptor, immobilized signal or imaging probe.
Use spacers when dye proximity affects hybridization, quenching, surface presentation or steric access.
Select compatible dark quenchers such as BHQ or QSY options based on reporter emission window.
Consider dye effects on duplex stability, probe length, GC content and background fluorescence.
Specify purity, mass confirmation and optional functional testing when signal performance is critical.
Bio-Synthesis supports multiple fluorescent labeling chemistries for terminal, internal and post-synthetic dye attachment workflows.
For 5′, 3′ or internal amino-modified oligos such as Amino-C6; forms stable amide linkages.
For thiol-modified oligos after reduction and desalting; useful for rapid, stable thioether dye attachment.
CuAAC, SPAAC and TCO–tetrazine strategies for modular dye placement and copper-free labeling workflows.
Aminooxy or hydrazide coupling for oxime or hydrazone-style dye attachment to carbonyl-bearing partners.
Direct solid-phase insertion for controlled position, spacing and reproducible fluorescent probe architecture.
Reporter/quencher, donor/acceptor and dual-labeled probe formats for qPCR, molecular beacon and FRET assays.
Fluorescent oligos are used across amplification, imaging, spatial biology, molecular detection and single-molecule workflows.
Hydrolysis probes, molecular beacons and multiplex reporter/quencher probe formats.
Donor/acceptor dye placement for distance-sensitive nucleic acid interaction studies.
Far-red and NIR dye-labeled oligos for low-background imaging and deeper wavelength detection.
Fluorescent oligo panels for chromosome painting, spatial biology and imaging workflows.
Channel-matched fluorescent oligos for probe-based plate, bead and multiplex detection systems.
Explore connected fluorescent probe and labeling services.
Include sequence, dye, position, assay type, instrument channel, scale and QC needs.
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