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Home » Antibody and Immunochemistry » Polyclonal Antibody Services » Select By Host Animals » Sheep/Goat Polyclonal Antibody

Custom Sheep or Goat Polyclonal Antibody

Bio-Synthesis (BSI) provides polyclonal antibody production in its USDA-licensed facility using Bohre Crossbred Goat or Suffolk Sheep that are both preconditioned and Q Fever Negative. These goats/sheeps often produce antibodies for larger batch volume. We can produce polyclonal antibody with a wide variety of antigens, from bacteria, viruses, fungi, fusion proteins, synthetic peptides, and plasmid DNA, as well as a variety of other proteins.



Typical antigen requirement is 5.0 mg conjugated peptide/animal, and 3.0 mg protein antigen/animal. However, amounts may vary due to antigen availability and additional antigen is required for affinity purification.

Two production schedules are provided for our goat/sheep polyclonal antibody services. Our standard procedure can be modified to customer specifications. ELISA determination results of antibody titer are faxed or e-mailed to you for your data package. Additional animals are available for all service packages.

BSI supplies a Polyclonal Antibody Information Sheet with each inquiry to assist in meeting our customer's specifications.
Don't see what you need? Just ask - in most cases we can accommodate your request. If you require help in choosing the services for your polyclonal antibody production, feel free to consult our experts.

All of our antibody packages can be prolonged with an additional immunization every 28 days which includes an additional bleed 10 days after the immunization. Production extension is an additional charge of animal maintenance fee.

Request a quote or contact us for your Sheep/Goat Polyclonal Antibody packages.
Service Descriptions Antigen Packages   Purification
 
Peptide
User
Supplied
Affinity Conjugation Protein
G
SS-PE-SG120 Super Class Standard (Sheep/Goat) Antibody Package: peptide synthesis >90%, conjugation, 70-90 √     √  
SP-PE-SG140G Super Class Plus G (Sheep/Goat) Antibody Package: peptide synthesis >90%, conjugation, protein G purification, 90-120 days √     √ √
SP-PE-SG140I Super Class Plus I (Sheep/Goat) Antibody Package: peptide synthesis >90%, conjugation, peptide affinity purification, 90-120 days √   √ √  
US-PR-SG120 User-Supplied Protein (Sheep/Goat) Antibody Package, 70-90 days   √      
US-PR-SG140G User-Supplied Protein G (Sheep/Goat) Antibody Package: Immunospecific affinity purification, 90-120 days   √     √
Two production schedules are available for each Sheep/Goat Polyclonal Antibody package along with its descriptions.

Available Production Time Frame:
  • 70-90 days
  • 90-120 days
Custom Peptide Polyclonal Antibody Services
Super Class Standard Peptide Antibody Package (Sheep/Goat)
Package: SS-PE-SG120 (70 Days Immunization Protocol) Time Frame 70-90 days and delivery
Peptide Synthesis > 90% (<20 a.a. 15 mg) ~1000 ml antiserum
KLH/BSA conjugation via Cysteine added on C- or N-term of peptide 3-5 mgs of unconjugated peptide
1 sheep/goat, 1 control sera, 1 initial inoculation, 4 boosters, 3 collection serum MS, HPLC of peptide and ELISA reports
ELISA titer assay on collection serum See immunization and delivery schedule.
 
Super Class Plus Protein G Column Purified Peptide Antibody Package (Sheep/Goat)
Package: SP-PE-SG140G (84 Days Immunization Protocol) Time Frame 90-120 days and delivery
Peptide Synthesis > 90% (<20 a.a. 15 mg) 200-800 mg protein G-puriified antibody from 100 ml antiserum
KLH/BSA conjugation via Cysteine added on C- or N-term of peptide ~900 mls of unpurified antiserum
1 sheep/goat, 1 control sera, 1 initial inoculation, 5 boosters, 1 test sera, 3 collection serum 3-5 mg of unconjugated peptide
Protein G purification purification of 100 ml antiserum MS, HPLC and ELISA assay reports
ELISA titer assay on collection sera and protein G purified sample Electronic image of SDS analysis
SDS PAGE analysis See immunization and delivery schedule.
 
Super Class Plus Immunoaffinity Purified Peptide Antibody Package (Sheep/Goat)
Package: SP-PE-SG140I (84 Days Immunization Protocol) Time Frame 90-120 days and delivery
Peptide Synthesis > 90% (< 20 a.a. 20 mg) 3-5 mg affinity purified antibody from 100 ml antiserum
KLH/BSA conjugation via Cysteine added on C- or N-term of peptide ~900 ml of unpurified antiserum
1 sheep/goat, 1 control sera, 1 initial inoculation, 5 boosters, 1 test sera, 3 collection serum 3-5 mgs of unconjugated peptide
Peptide immobilization on sepharose beads MS, HPLC of peptide
Loading of the antiserum on the affinity column and elution of bound antibodies (100 ml antiserum) ELISA assay reports
ELISA titer assay on crude collection serum and peptide immunopurified antibodies Electronic image of SDS analysis
SDS PAGE analysis See immunization and delivery schedule.
 
User Supplied Immunogen - Peptide/Recombinant and Fusion Protein
User-Supplied Protein Antibody Package (Goat or Sheep)
Package: US-PR-SG120 (70 Days Immunization Protocol) Time Frame 70-90 days and delivery
Conjugation to carrier (optional, additional fee) ~1000 ml antiserum
1 sheep/goat, 1 control sera, 1 initial inoculation, 4 boosters, 3 collection serum ELISA assay report
ELISA titer assay of collection serum See immunization and delivery schedule.
Antigen requirement: 3.5-4.0 mgs of protein. The concentration should be 1 mg/ml is a good target range, minimum >0.5 mg/ml in PBS buffered solution pH 7.4.
See details on antigen preparation
 
User-Supplied Protein G Purified Antibody Package (Goat or Sheep)
Package: US-PR-SG140G (84 Days Immunization Protocol) Time Frame 90-120 days and delivery
Conjugation to carrier (optional, additional fee) 200-800 mg protein G purified antibody from 100 ml of antiserum
1 sheep/goat, 1 control sera, 1 initial inoculation, 5 boosters, 1 test sera, 3 collection serum ~900 ml unpurified antiserum
Protein G purification ELISA assay report
ELISA titer assay of collection serum and protein G purified sample Electronic image of SDS analysis
SDS-PAGE analysis See immunization and delivery schedule.
Antigen requirement: 3.5-4.0 mgs of protein. The concentration should be 1 mg/ml is a good target range, minimum >0.5 mg/ml in PBS buffered solution pH 7.4.
See details on antigen preparation
 
We offer two standard custom antibody protocols (combinations of hosts and protocol time frame) From peptide design, synthesis, and conjugation, to antibody generation and purification, Bio-Synthesis offers a complete package to meet your protein detection needs. User-design production protocol also available and can also be modified from existing package.
Serum Production Specification:
Immunization schedule listed below are approximate (± 2 days).

  • Two Boer, Nubian, Alpine or ToggenbergCrossbred Goat or Suffolk will be utilized for each MAP- peptide, conjugated-peptide immunogen, or protein immunogen packages.
  • Total material required for injection for the program below is 3.0-3.5 mg protein/animal, 6.0-7.0 mg peptide conjugate/animal. If we are conjugating your protein we assume a 20% loss of protein during conjugation. Total amount of serum expected from a goat/sheep on the program below is approximately ~1000 mL/animal.
  • Immunogen is emulsified in Freund's Complete Adjuvant (FCA) for initial injections. Freund's Incomplete Adjuvant (FIA) is used for all subsequent injections (boosts).
  • Continuation of project past our standard immunization schedule is offered on a month to month basis. The standard extension protocol includes one booster, two serum collections, and maintenance for one month. Terminal bleeds may be obtained at any time yielding approximately ~1000 ml/animal of additional plasmaphoresis serum taken upon customer request.
 
Program Development of Sheep/Goat Polyclonal Antibody: 70-90 Days
Procedure Protocol Days* Description
Phase I - Antigen design, synthesis and preparation
Antigen Preparation ~5 - 10 days Peptide synthesis and purification (if required) and antigen conjugation
Phase II - Immunization Schedule

Control sera collection

Initial Inoculation		 Day 0 Boer, Nubian, Alpine or Toggenberg Crossbred Goat or Suffolk and Control sera (~10 ml serum/host)

Initial Inoculation with 2.0 mg peptide conjugate or 1.0 mg protein per host with FCA in SQ 20 sites
1st Booster Day 14 Boost with 1.0 mg peptide conjugate or 0.5 mg protein per host with FIA in SQ 20 sites
2nd Booster Day 28 Boost with 1.0 mg peptide conjugate or 0.5 mg protein with FIA in SQ 20 sites
1st Serum Collection

3rd Booster		 Day 42 Week 6 Collection sera ~300 ml/host

Boost with 1.0 mg peptide conjugate or 0.5 mg protein per host with FIA in SQ 20 sites
2nd Serum Collection

4th Booster

ELISA Titer Assay		 Day 56-63 Week 8 Collection sera ~300 ml/host

Boost with 1.0 mg peptide conjugate or 0.5 mg protein per host with FIA in SQ 20 sites

ELISA assay of control, week 6, 8 collection serum
1st Shipment

Project Completion Verification***		 Day 64-65 1st shipment of control, week 6 and 8 collection serum

Verify whether the program should be continued, modified, or terminated.
3rd Serum Collection

2nd Shipment		 Day 70 Week 10 Collection sera ~300 ml/host

2nd shipment of week 10 collection serum
Instruction Due Date Day 84 Termination of animals if instruction for extension or termination not received
*** contact us for further details regarding project completion.
 
Program Development of Sheep/Goat Polyclonal Antibody: 90-120 Days
Procedure Protocol Days* Description
Phase I - Antigen design, synthesis and preparation
Antigen Preparation ~5 - 10 days Peptide synthesis and purification (if required) and antigen conjugation
Phase II - Immunization Schedule
Control sera collection



Initial Inoculation
Day 0 Boer, Nubian, Alpine or Toggenberg Crossbred Goat or Suffolk and Control bleeds (~10 ml serum/host)

Initial Inoculation with 2.0 mg peptide conjugate or 1.0 mg protein per host with FCA in SQ 20 sites
1st Booster Day 14 Boost with 1.0 mg peptide conjugate or 0.5 mg protein per host with FIA in SQ 20 sites
2nd Booster Day 28 Boost with 1.0 mg peptide conjugate or 0.5 mg protein with FIA in SQ 20 sites
Test Sera Collection

3rd Booster
 Day 42 Test Sera Collection ~10-15 ml/host

Boost with 1.0 mg peptide conjugate or 0.5 mg protein per host with FIA in SQ 20 sites
1st Serum Collection

4th Booster		 Day 56 Week 8 Collection sera ~300 ml/host

Boost with 1.0 mg peptide conjugate or 0.5 mg protein per host with FIA in SQ 20 sites

2nd Serum Collection

5th Booster

ELISA Titer Assay
 Day 70-75 Week 10 Collection sera ~300 ml/host

Boost with 1.0 mg peptide conjugate or 0.5 mg protein per host with FIA in SQ 20 sites

ELISA assay of control, week 6 test sera, week 8 collection sera

1st Shipment

Project Completion Verification***		 Day 76-77 1st shipment of control, week 6 test sera, week 8 collection sera

Verify whether the program should be continued, modified, or terminated.
3rd Serum Collection

2nd Shipment
 Day 84-90 Week 12 Collection sera ~300 ml/host

2nd shipment of week 12 collection sera
Instruction Due Date Day 98 Termination of animals if instruction for extension or termination not received
Phase III - Post Production Sample Processing
Serum Processing

ELISA and/or SDS Analysis

3rd (Final) Shipment		 ~ 2 weeks Affinity column preparation, purification and/or labeling

Analysis of purified sample

Shipping of purified sample, unpurified week 12 sera and documents
*** contact us for further details regarding project completion.
Links and Resources

Forms:
Order/Quote Page

Additional Phospho-Specific Antibody Information:
Phospho-Specific Life-Cycle Illustration

Sci Apps:
Peptide Property Calculator
Peptide Design Library
Oligo Designer

Useful Links
Reference/Citing:
1.
Different prelamin A forms accumulate in human fibroblasts: a study in experimental models and progeria
2.
1,25-DIHYDROXYVITAMIN D3-INDUCED GENES IN OSTEOBLASTS: UNCOVERING NEW FUNCTIONS FOR MENINGIOMA 1 AND SEMAPHORIN 3B IN SKELETAL PHYSIOLOGY
3.
Fetal Death in Mice Lacking 5α-Reductase Type 1 Caused by Estrogen Excess
4.
A splicing variant of a death domain protein that is regulated by a mitogen-activated kinase is a substrate for c-Jun N-terminal kinase in the human central nervous system
Ordering Services
To place an order or request a quotation, please proceed to our custom Order/Quote page or Contact Us for any other questions.
Order can be placed with either a PO (Purchase Order) or credit card. You may place order online using our secure online ordering system. Our customer service representatives are available 24 hours a day, Monday through Friday, to assist you.
   
We accept POs and major credit cards ( ). Your credit card will be billed under "Bio-Synthesis, Inc." Please complete and return our Business Credit Application. For international orders, we must apply the full charge at the time of the order is placed. In the unlikely event that any given order cannot be filled, our guarantee will take the form of a full refund.
 
 
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