Custom siRNA Synthesis - Road to gene silencing
Synthetic siRNA and shRNA constructs are two of the numerous options available. BSI provides the custom synthesis of
sequence-specific siRNA for direct transfection, as well as the routine synthesis
of shRNA oligos, ~60 bases long purified DNA oligos to be cloned to form shRNA constructs
. Our siRNAs (small interfering RNAs) are double-stranded RNA molecules, made of
19 RNA nucleotides with two nucleotides (usually DNA bases) 3' overhangs. They are
used to knockdown a specific gene of interest for identifying gene function, elucidating
pathways and screening for potential new drug targets.
Looking for a reliable source for your RNAi that
offers.
Rapid turn-around time and high throughput capacity
Modified nucleotides now available for siRNA products
Deprotected, desalted, annealed and ready-to-use
µg to multi-gram capacity for ASR or cGMP grade
Available in duplex or single-stranded form
Modifications and Labelings
Custom siRNA is available with a variety of fluorescent labels and other modifications.
Alexa Fluor dyes provide superior performance and can be directly substituted for
other common dyes. Other fluorescent dyes available include fluorescein,
rhodamine, Cy3, and Cy5. Custom siRNA may be labeled at either the 3' or the 5'
end of the sense strand, and labels do not affect the biological activity of the
siRNA. As well as fluorescent labels, many other backbone and base modifications
are available including amino linkers, thio linkers, biotin, dabcyl and phosphate
modifications.
View the
full range of modification options Special modifications are also
available on request; please contact our
RNA oligo Technical Specialists.
All siRNA oligos are provided as a lyophilized pellet to ensure stability during
delivery.
Synthesis Platform
Bio-Synthesis provides siRNA by using proprietary synthesis and purification processes
with final products that are ready-to-use. There is no need for further time-consuming
and expensive HPLC or PAGE purification. The high-throughput synthesis and purification
processes enable fast turnaround times and high yields. Each duplex siRNA synthesis
undergoes stringent quality control including MALDI-TOF mass spectrometry analysis
that verifies identity of the component strands and analytical HPLC QC that verifies
purity of the duplex itself, a level of QC unparalleled in the industry. Our high
performance grade siRNA is economically priced allowing the use of highly pure siRNA
in all routine RNAi experiments. For very sensitive applications, custom synthesis
of HPLC-purified siRNA is also available upon request. Read more about siRNA synthesis
platform and technology.
The most trusted name in Quality Custom Synthesis