siRNA Synthesis

• Desalted Single-Stranded:

1. Oligos are shipped deprotected, desalted single strand in a separate tube.
2. Dried duplexes are provided in the absence of universal buffer salts.
3. Oligos are ready for use immediately upon resuspension.
4. Estimated shipping time is 4 business days for orders received before 3:00pm CST. Ship date will be confirmed upon order placement.

• Desalted Duplex strands:

1. Duplex oligos are shipped deprotected and desalted.
2. Dried duplexes are provided in the absence of universal buffer salts.
3. The oligos are ready for use immediately upon resuspension.
4. Estimated shipping time is 4 business days for orders received before 3:00pm CST. Ship date will be confirmed upon order placement.

• HPLC Purified:
  Please Note: The siRNA duplex is now dried down in the absence of buffer and the amount packaged per tube has changed, however, the total amount of material supplied remains the same.
1. Oligos are shipped deprotected, duplexed, purified, and desalted.
2. Dried duplexes are provided in the absence of universal buffer salts, unless otherwise specified upon ordering.
3. The oligos are ready for use immediately upon resuspension.
4. Typical shipping time is 6 business days for orders received before 3:00pm CST. Ship date will be confirmed upon order placement.

• siRNA In-Vivo-Ready: A minimum of 90% purity. These duplexes are shipped deprotected with extra processing which also includes counter ion (Na+) exchange, dialysis, sterile filtration and endotoxin testing for the introduction of siRNAs into animals.

  Estimated shipping time is 10 business days for orders received before 3:00pm CST. Ship date will be confirmed upon order placement.

• HPLC purified: In-Vivo-Ready custom synthesized siRNAs provide the highest quality siRNAs available anywhere with guaranteed purity of 97%. Pprocessing includes an extra level of HPLC purification, counter ion (Na+) exchange, dialysis to remove salt (to <1 microsiemens conductance at 50 µM siRNA), sterile filtration and endotoxin tested.

  Estimated shipping time is 20 business days for orders received before 3:00pm CST. Ship date will be confirmed upon order placement.

Custom siRNA is available with a variety of fluorescent labels and other modifications. Alexa Fluor dyes provide superior performance and can be directly substituted for other common dyes. Other fluorescent dyes available include fluorescein, rhodamine, Cy3, and Cy5. Custom siRNA may be labeled at either the 3' or the 5' end of the sense strand, and labels do not affect the biological activity of the siRNA. As well as fluorescent labels, many other backbone and base modifications are available including amino linkers, thio linkers, biotin, dabcyl and phosphate modifications. See the full range of our modification options. Special modifications are also available upon request. You may contact us for additional information.

Note: all siRNA oligos are provided as lyophilized pellets to ensure stability during delivery.

Bio-Synthesis provides siRNA by through proprietary synthesis and purification processes with final products that are ready-to-use. There is no need for further time-consuming and expensive HPLC or PAGE purification. The high-throughput synthesis and purification processes enable fast turnaround and high yields. Each duplex siRNA synthesis undergoes stringent quality control including MALDI-TOF mass spectrometry analysis that verifies identity of the component strands and analytical HPLC QC to verify purity of the duplex itself, a level of QC unparalleled in the industry. Our high performance grade siRNA is economically priced allowing the use of highly pure siRNA in all routine RNAi experiments. For very sensitive applications, custom synthesis of HPLC-purified siRNA is also available upon request.

Standard QC package

Analytical anion-exchange HPLC of oligonucleotide; purity guaranteed above 85% in case purification is required. TOF/MS for oligonucleotide identification.

Advanced QC package

Standard QC package and additionally:

Ion-pair reverse phase HPLC of the oligonucleotide at 60^oC, purity guaranteed above 85% analytical reverse phase HPLC Endotoxin analysis of the oligonucleotide (*phosphorothioates may inhibit in the mechanism for endotoxin determinations Bio- Synthesis is using and therefore reading might be less accurate for these molecules) pH determination of a 1% oligonucleotide solution.

Custom QC Package:

Determination of the sodium content of the oligonucleotide (minimum 20 mg of purified oligonucleotide is required). LC-MS of the oligonucleotides. Any custom quality control methods could be developed