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Home » Antibody and Immunochemistry » Polyclonal Antibody Services » Select By Service Packages » Single Peptide Polyclonal Antibody
 
Single Peptide Polyclonal Antibody Services
 
Single Peptide Polyclonal Antibody Services

Custom Polyclonal Antibody Services

Polyclonal antibody generates from synthetic peptides or protein have become an important reagents to be used as both primary and secondary antibodies in Westerns, ELISAs, and IHCs. Bio-Synthesis' staff are experts in the use of synthetic peptide antigens to target specific antigenic epitopes. All of our custom anti-peptide polyclonal antibody services include peptide design by using our proprietary Antigen IdentifierTM Design Tool to select immunogenic peptide sequences. The overall aim is to identify regions of the protein that are most likely to be accessible on the surface of the full length protein. Furthermore, all procedures performed during immunization are described in our Standard Operation Procedures (SOPs) to ensure the highest level of quality and service.

Benefits of BSI Polyclonal Antibody Servces

  • Dedicated project manager
  • Free pepitde antigen design
  • Peptide synthesis and conjugation
  • Flexible immunization and bleed protocol
  • Variety of hosts in accredited animal facilities
  • Antibody purifications (optional)
  • QC by ELISA, SDS analysis, Western blotting (optional)
  • Guaranteed results

BSI supplies a Polyclonal Antibody Information Sheet with each inquiry to assist in meeting customer specifications.
Bio-Synthesis offers 3 different types of polyclonal antibody packages using 4 different types of production schedules depending on your research goals and budgets. We welcome you to explore the various packages or to create your own.

Request a quote or contact us for your Single Peptide Polyclonal Antibody packages.

The Immunoaffinity Purified - First Class Plus Peptide Polyclonal Antibody Package

Package: FP-PE-RA120I (84 Days Immunization Protocol) Time Frame 90-120 Days and Delivery
Peptide Synthesis > 80% (<20 a.a. 15 mg) ~8 ml control serum
KLH/BSA conjugation via Cysteine added on C- or N-term of peptide up to 25 mg of peptide affinity purified antibody from 25 ml antiserum
2 rabbits, 2 control serum (1/animal), 2 initial inoculations (1/animal), 10 boosters (5/animal), 2 test serum (1/animal), 6 production serum (3/animal) Remaining unpurified antiserum
Peptide immobilization on sepharose beads 5 mg of unconjugated peptide
Loading of the antiserum on the affinity column and elution of bound antibodies MS, HPLC of peptide, ELISA reports
ELISA titer assay on crude collection sera and peptide immunopurified antibodies Electronic Image of SDS analysis
SDS Page Analysis See immunization and delivery schedule

Super Class Plus M Immunospecific Peptide Antibody - Western Guaranteed!

Package: SP-PE-RA120M (84 Days Immunization Protocol) Time Frame 90-120 Days and Delivery
Peptide Synthesis > 90% (<20 a.a. 15 mg) ~8 ml control serum
KLH/BSA conjugation via Cysteine added on C- or N-term of peptide up to 25 mg peptide affinity purified antibodies from 25 ml antiserum
2 rabbits, 2 control serum (1/animal), 2 initial inoculations (1/animal), 10 boosters (5/animal), 2 test serum (1/animal), 6 production serum(3/animal) Remaining unpurified antiserum
Peptide immobilization on sepharose beads Peptide affinity purified column
Loading of the antiserum on the affinity column and elution of bound antibodies 5 mg of unconjugated peptide
ELISA titer assay on crude collection sera and peptide immunopurified antibodies MS, HPLC of peptide, ELISA reports
Western Blot analysis on purified antibody Electronic Image of the blot is provided
4 lysate panels See immunization and delivery schedule

Antipeptide Polyclonal Antibody Packages

Packages Species Peptide Synthesis Conjugation Purification
     
>70 >80 >90
KLH
Affinity Protein
Affinity IgY
A G
Economy Express Peptide Ab Rabbit √     √        
Economy Standard Peptide Ab √     √        
First Class Standard Peptide Ab   √   √        
First Class Plus Protein A Purified Peptide Ab   √   √     √  
First Class Plus Immunoaffinity Purified Peptide Ab   √   √ √      
Super Class Standard Peptide Antibody (70-90 days)     √ √        
Super Class Plus Protein A Purified Peptide Ab     √ √     √  
Super Class Plus Immunoaffinity Purified Peptide Ab     √ √ √      
Super Class Plus M Western Characterized Peptide Ab     √ √ √   √  
First Class Standard Peptide Ab Guinea Pigs   √   √        
First Class Plus Protein A Peptide Ab   √   √     √  
First Class Plus Immunoaffinity Purified Peptide Ab   √   √ √      
Super Class Standard Peptide Ab     √ √        
Super Class Plus Protein A Purified Peptide Ab     √ √     √  
Super Class Plus Immunoaffinity Purified Peptide Ab     √ √ √      
Super Class Standard Peptide Ab Mouse     √ √        
Super Class Plus Protein A Column Purified Peptide Ab     √ √     √  
Super Class Standard Peptide Ab Rat     √ √        
Super Class Plus Protein G Column Purified Peptide Ab     √         √
Super Class Peptide Plus IgY Purified Ab Chicken     √ √   √    
Super Class Peptide Combo Immunoaffinity and IgY Purified     √ √ √ √    
Super Class Standard Peptide Antibody Package Sheep/Goat     √ √        
Super Class Plus Protein G Column Purified Peptide Ab     √ √       √
Super Class Plus Immunoaffinity Purified Peptide Ab     √ √ √      
Antibody derived from synthetic peptide or protein have become an important tools in life science research and diagnostic for protein detection and quantification. Applications include:

  • Protein processing studies
  • Diagnostic tests
  • Protein localization
  • Active site determination
  • Gene product detection and identification
The major challenge encountered when preparing peptide antibodies is their ability to eliciting an immune response and recognize the native protein. Key to success is a proper selection of the peptides used in the immunization process. All of our custom anti-peptide polyclonal antibody services include peptide design by using our proprietary Antigen IdentifierTM Design Tool to select immunogenic peptide sequences. with most advanced algorithms. Each peptide antigen is measured against several protein databases to confirm the desired antibody and epitope specificity. The overall aim is to identify regions of the protein that are most likely to be accessible on the surface of the full length protein. We typically try to suggest three potential peptide candidates to choose from.

The single peptide strategy is best suited for reproducing the previously reported success of a polyclonal production with a known peptide sequence.
Our peptide design strategies
Our Antigen IdentifierTM Design Tool searches and accesses your sequence to identify the best candidate peptides that optimize synthesis success, solubility and antigenicity. Each peptide is compared with BLAST databases of over two dozen species to limit cross-reactivity while improving overall antibody specificity. When examining a protein sequences for potential antigenic epitopes, Our Antigen Identifier program assist you with in predicting protein characteristics such as:
  • Domain exclusion analysis
  • Hydrophobicity and antigenicity analysis
  • Homology search
  • Targeting the N-terminus or C-terminus
  • Continuous versus Discontinuous Epitopes
  • Peptide candidates
  • Coupling strategy
  • Expertise
How to submit peptide antibody design request?
Our peptide antibody design service is free of charge when placing an order for a custom polyclonal antibody project. Please contact us online and provide us with following information:
  • Project Name
  • Accession Number
  • Region: You may specify regions which should be avoided or targeted, by entering one or more intervals separated by commas: e.g. 1-150, 250-350
  • Region Options: Indicate whether the regions specified should be avoided or targeted
  • Protein Sequence: This is the sequence we should search for suitable peptide sequences in the absence of an accession number.
  • Comments: Questions, additional pieces of information, clarifications and comments accompanying an order for peptide design. Please feel free to be verbose and enter as much information as you wish.
Animal Production Schedules

Sample Schedules of Events using Peptide Immunogens
Animal Antigen Required Per Host Estimated Serum Volume/Host
Rabbit Peptide, 56-70 Day [EXPRESS] 2mg ~ 50 mL
Rabbit Peptide, 70-90 Day 2mg ~ 75 mL
Rabbit Peptide, 90-120 Day 2 mg plus additional antigen for purification ~ 75 mL
Guinea Pig Peptide, 70-90 Day 2mg 19mL
Guinea Pig Peptide, 90-120 Day 2 mg plus additional antigen for purification 19mL
Rat Peptide, 70-90 Day 1.5mg 15.5mL
Rat Peptide, 90-120 Day 1.5mg 15.5mL
Mouse Peptide, 70-90 Day 1.0mg 0.8mL
Mouse Peptide, 90-120 Day 1.0mg 0.8mL
Chicken Peptide, 90-120 2.0 mg plus additional antigen for purification 18 mL***
Goat/Sheep Peptide, 90-120 5.0 mg plus additional antigen for purification 1000mL
Goat/Sheep Peptide, 120-140 5.0 mg plus additional antigen for purification 1000mL

Sample Schedules of Events using Protein Immunogens
Animal Antigen Required Per Host Estimated Serum Volume/Host
Rabbit Protein, 56-70 Day [EXPRESS] 1.0mg ~ 50 mL
Rabbit Peptide, 70-90 Day 1.0mg ~ 75 mL
Rabbit Peptide, 90-120 Day 1.0 mg plus additional antigen for purification ~ 75 mL
Guinea Pig Peptide, 70-90 Day 1.0mg 19mL
Guinea Pig Peptide, 90-120 Day 1.0 mg plus additional antigen for purification 19mL
Rat Peptide, 70-90 Day 800 µg 15.5mL
Rat Peptide, 90-120 Day 800 µg 15.5mL
Mouse Peptide, 70-90 Day 500 µg 0.8mL
Mouse Protein, 90-120 Day 500 µg 0.8mL
Chicken Peptide, 90-120 1.0 mg plus additional antigen for purification 18 mL***
Goat/Sheep Peptide, 90-120 3mg 1000mL
Goat/Sheep Peptide, 120-140 5.0 mg plus additional antigen for purification 1000mL
**Eggs yield 40-100 mg IgY per egg, ~5-7 eggs per week
*Additional animals available for all protocols.
All of our antibody program can be prolonged with an additional immunization every 28 days, and an additional bleed 10 days after the immunization. Production extension is an additional charge of animal maintenance fee.
Links and Resources

Forms:

Order/Quote Page

Protein Analysis Service for Antigenic Peptides

Additional Phospho-Specific Antibody Information:
Phospho-Specific Life-Cycle Illustration

Sci Apps:
Peptide Property Calculator
Peptide Design Library
Oligo Designer

Useful Links
Reference/Citing:
1.
Masaru Yamamoto, et al. "Phosphorylation of Claudin-5 and Occludin by Rho Kinase in Brain Endothelial Cells" The American Journal of Pathology, Vol. 172, No. 2, February 2008
2.
Rakesh K. Singh, et al. "Olanzapine Increases RGS7 Protein Expression via Stimulation of the Janus Tyrosine Kinase-Signal Transducer and Activator of Transcription Signaling Cascade" The Journal of Pharmacology and Experimental Therapeutics, Vol. 322, No1
3.
Ying Zhou, et al. "Growth control of multiple myeloma cells through inhibition of glycogen synthase kinase-3" , Leukemia and Lymphoma, Volume 49, Issue 10 October 2008 , pages 1945 - 1953
4.
Peter D. Adams, "Retinoblastoma Protein Contains a C-terminal Motif That Targets It for Phosphorylation by Cyclin-cdk Complexes", Molecular and Cellular Biology, February 1999, p. 1068-1080, Vol. 19, No. 2
Ordering Services
To place an order or request a quotation, please proceed to our custom Order/Quote page or Contact Us for any other questions.
Order can be placed with either a PO (Purchase Order) or credit card. You may place order online using our secure online ordering system. Our customer service representatives are available 24 hours a day, Monday through Friday, to assist you.
   
We accept POs and major credit cards ( ). Your credit card will be billed under "Bio-Synthesis, Inc." Please complete and return our Business Credit Application. For international orders, we must apply the full charge at the time of the order is placed. In the unlikely event that any given order cannot be filled, our guarantee will take the form of a full refund.
 
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