Enhanced Diagnostic Tools
Acridine is an effective intercalating agent as well as being a lipophilic carrier molecule. Acridine labeled oligonucleotides are sometimes used in antisense research. Oligos containing acridine cannot be HPLC purified, nor can they be analyzed by HPLC, as they bind irreversibly to the column. Acridine is available as both a 3' and a 5' modifier. Labeling is performed during synthesis and is not a benchtop reaction.
Desalt or cartridge (RP1) purification is acceptable for most common phosphoramidite modifiers. However, additional purification is strongly recommended for modifiers that require NHS-ester chemistry to conjugate a dye through an amine linker such as Molecular Probes dyes.
Due to the chemistry of many modifications, yield will be approximately 40 - 60 % less than a standard oligo. Please see our yield chart for details.
Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields are determined using UV absorbance at OD260 In addition, we perform QC methods tailored to specific modifications, such as OD ratio measurement where appropriate.
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