Enhanced Diagnostic Tools
New set of excellent fluorescent dyes from ATTO-Tec has added to our broad portfolio of labels for oligonucleotides. These activated fluorescent dyes have become the standard procedure for stable labelling of proteins, nucleic acids, ligands and other biomolecules, which are widely used for applications as fluorescence microscopy, flow cytometry, fluorescence in situ hybridisation (FISH), receptor binding assays, or enzyme assays.
ATTO dyes are a series of fluorescent dyes which provide all the crucial properties required for modern fluorescent technologies.
This compound requires amino- or thiol- linker with a 6-Carbon spacer arm or by substituting any Amino Modifier C6 dA, dT, dG, dC for internal labeling, a double HPLC purification is required.
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A wide variety of modifications can be incorporated into an oligonucleotide at the time of synthesis, using aspecialized phosphoramidite chemistries for labeling. However, certain modifications require the use of post-synthetic conjugation strategies in which a primary amine modified oligonucleotide is covalently attached to a dye via NHS ester chemistry. Other cross-linking chemistries are also available depending on particular project specifications. Single HPLC purification is recommended when using phosphoramidite oligo chemistry, otherwise dual HPLC is required for post-synthetic labeling.
Due to the chemistry of many modifications, yield will be approximately 40 - 60 % less than a standard oligo. Please see our yield chart for details.
Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields are determined using UV absorbance at OD260. In addition, we perform QC methods tailored to specific modifications, such as OD ratio measurement where appropriate.
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