The most accurate Tm value can be obtained in the range from 9 to 20 nucleotides. It is, however, possible to obtain reasonably accurate Tm values in other ranges.
The predicted Tm values are based on the following:
- Salt concentration (sodium ion) from 100 to 150 mM.
- Neutral or closely neutral solution (pH 7.0 - 8.3).
- Oligonucleotide concentrations between 2 and 4 uM.
Note 1: Due to the limitation of RNA hybridization information, the RNA Tm values may not be precise.
Note 2: This tool is based on a modified nearest-neighbor thermodynamic model and experimental data.