Enhanced Diagnostic Tools
Bodipy® Dyes have a narrow spectral characteristics and are highly fluorescent
that are often superior to those of fluorescein, tetramethylrhodamine, Texas Red
and longer-wavelength dyes. These dyes are unusual in that they are:
Bodipy labels are attached at the 5'- , Internal, or 3'- end of an oligonucleotide.
In a post-synthetic step, the dye is coupled to an amino-modified oligo.
This compound requires amino- or thiol- linker with a 6-Carbon spacer arm or by
substituting any Amino Modifier C6 dA, dT, dG, dC for internal labeling, a double
HPLC purification is required.
Contact your Account Representative for pricing on BODIPY labeled oligonucleotides. For additional information, please
Price/labeling, purification and synthesis is an additional charge
A wide variety of modifications can be incorporated into an oligonucleotide at the time of synthesis, using aspecialized phosphoramidite chemistries for labeling. However, certain modifications require the use of post-synthetic conjugation strategies in which a primary amine modified oligonucleotide is covalently attached to a dye via NHS ester chemistry. Other cross-linking chemistries are also available depending on particular project specifications. Single HPLC purification is recommended when using phosphoramidite oligo chemistry, otherwise dual HPLC is required for post-synthetic labeling.
Due to the chemistry of many modifications, yield will be approximately 40 - 60
% less than a standard oligo. Please
see our yield chart for details.
Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF
mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final
yields are determined using UV absorbance at OD260. In addition,
we perform QC methods tailored to specific modifications, such as OD ratio measurement
For additional information, please contact us.