C3 Spacer Oligonucleotide Modification
Bio-Synthesis offers C3 Spacer oligonucleotide modification at various scales.
The C3 propyl spacer can be incorporated internally or at the either end of the
oligo during chemical synthesis. Multiple C3 spacers can be added to introduce a
longer spacer arm for the attachment of fluorophores or other pendent groups.
Spacer C3-modified oligos have been used in a number of different applications,
including protein-RNA functional studies1 , as a DNA abasic site mimic
to study the utility of small synthetic ligands (such as pteridines) for nucleotide
recognition in SNP typing applications 2, and for solid-phase immobilization
of hybridization probes3 . Spacer C3 incorporated at the 3’-end of an
oligo functions as an effective blocking agent against polymerase extension at that
end in PCR reactions4
Contact Bio-Synthesis for C3 Spacer Oligonucleotide
Modification Service.
Purification:
Spacer modified oligonucleotide can be accomplished by various methods. Desalting
or cartridge purification is acceptable ,however, additional purification by HPLC
is strongly recommended.
Price:
Price/modification. 10% discount for more than two spacer modifications.
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5' C3 Spacer Modifications
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100 nmole DNA oligo
250 nmole DNA oligo
1 µmole DNA oligo
5 µmole DNA oligo
10 µmole DNA oligo
100 nmole RNA oligo
250 nmole RNA oligo
1 µmole RNA oligo
5 µmole RNA oligo
10 µmole RNA oligo
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$45.00
$55.00
$85.00
$150.00
$300.00
$45.00
$55.00
$85.00
$150.00
$300.00
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Internal C3 Spacer Modifications
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100 nmole DNA oligo
250 nmole DNA oligo
1 µmole DNA oligo
5 µmole DNA oligo
10 µmole DNA oligo
100 nmole RNA oligo
250 nmole RNA oligo
1 µmole RNA oligo
5 µmole RNA oligo
10 µmole RNA oligo
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$45.00
$55.00
$85.00
$150.00
$300.00
$45.00
$55.00
$85.00
$150.00
$300.00
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3' C3 Spacer Modifications
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100 nmole DNA oligo
250 nmole DNA oligo
1 µmole DNA oligo
5 µmole DNA oligo
10 µmole DNA oligo
100 nmole RNA oligo
250 nmole RNA oligo
1 µmole RNA oligo
5 µmole RNA oligo
10 µmole RNA oligo
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$45.00
$55.00
$85.00
$150.00
$300.00
$45.00
$55.00
$85.00
$150.00
$300.00
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References:
- Pritchard, C.E., Grasby, J.A., Harny, F., Zacharek, A.M., Singh, M., Karn, J.,
Gait, M.J. Methylphosphonate mapping of phosphate contacts critical for RNA recognition
by the human immunodeficiency virus tat and rev proteins. Nucleic Acids Res. (1994),
22: 2592-2600.
- Dai, Q., Xu, C-Y., Sato, Y., Yoshimoto, K., Nishizawa, S., Teramae, N. Enhancement
of the Binding Ability of a Ligand for Nucleobase Recognition by Introducing a Methyl
Group. Anal. Sci. (Japan) (2006), 22: 201-203.
- Li, H., McGall, G. Photoactivatable Silanes: Synthesis and Uses in Biopolymer
Array Fabrication on Glass Substrates. In Frontiers in Biochip Technology, X, W-L.,
Cheng, J. (Ed.). Springer Science+Business Media, Inc. (2006). pp. 176-190.
- Zhou, L., Myers, A.N., Vandersteen, J.G., Wang, L, Wittwer, C.T. Closed-Tube
Genotyping with Unlabeled Oligonucleotide Probes and a Saturating DNA Dye. Clin.
Chem. (2004), 50: 1328-1335.