Sample Preparation Products

Home » Catalog » Sample Preparation Products » DNA and RNA Ladders » RNA Ladders and Markers » 1 kb RNA Ladder

1 kb RNA Ladder

Catalog#:

NB-15003

Price/Unit: $135

Unit:

50 loads

Bio-Synthesis 1kb RNA ladder is a set of RNA transcripts derived from recombinant DNA templates. This ladder is suitable for precise sizing of a wide range of RNA molecules using native or denaturing agarose gels, and can be visualized under UV.

Nine discrete bands, ranging from 200 to 4,000 bases
Highly stable
No need for staining or destaining as loading dye contains ethidium bromide
Convenient lyophilized format provides better product stability

Contents

2 vials of lyophilized RNA ladder (25 loads each).
1x loading buffer: 33.3% formamide, 11.7% formaldehyde, 0.012% bromophenol blue, 0.05mM EDTA, 0.012% ethidium bromide, and 0.7x MOPS solution.
2x loading buffer. I

Instructions:

To reconstitute the lyophilized RNA ladder, add 250 µL of 1x loading buffer to each 25 loads vial and vortex gently.
Heat at 80°C for 10 minutes. Incubate on ice for 1 min. Load 10 µL on a 1.5-2% gel.
For optimal results, use BSI 2x loading buffer with each RNA sample. There is no need for staining and destaining denaturing gels since Norgen’s loading buffer contains ethidium bromide.

Storage:

Store at -20°C.