Long RNA Oligo by Transcription

In Vitro Transcribed Long RNA

The ability to synthesize target RNA, such as mRNA, in the laboratory is critical to certain molecular biology techniques and genomic studies including ribozyme and aptamer synthesis, RNA structure and processing analyses, RNA interference and antisense RNA techniques. Bio‑Synthesis is the only company that offers the full range of services to manufacture high quality ultra long RNA synthetically or by in vitro transcription from template options including Plasmids, PCR Products, oligonucleotides and cDNA. Whether you need research-grade RNA, a control template for molecular assays or a modified capped RNA, Bio‑Synthesis has the track record and expertise in providing high quality RNA, along with flexibility which is unmatched by other suppliers.

All d2RNA^TM, in vivo transcription RNA, are delivered fully purified using various purification options and quality checked including, but not limit to, DNA sequencing, DNA, RNA size checked by electrophoresis, or real-time PCR, qPCR analysis. Custom assay development and validation are also available upon request.

Final RNA oligos are delivered normalized and packaged lyophilized in an inert chemical matrix that inactivates trace RNase in the liquid phase, providing an added level of RNA stability during sample handling, with or without ice.

Description Quality Services Resources How to Order

Long RNA by Transcription

While there has been significant demand for ultra long RNA oligonucleotides for gene structure and functional studies, the synthesis of long RNA fragments using the conventional RNA synthesis method is highly sequence-dependent. This is due to the potential for secondary structure formation and the inherent difficulty for chemical synthesis with additional 2'-OH reactive group of RNA introduces considerable complexity to the synthesis. Usually the conventional method can only synthesize RNA fragments under 100 bases. Bio‑Synthesis has met the challenge of synthesizing thousands of semi-synthetic long RNA, d2RNA^TM (3000 bases and more) using the best reagents available, proprietary methods to optimize the RNA manufacturing process, and the expertise in nucleic acid chemistry and molecular biology since 1984.

Each in vitro transcribed long RNA oligo is meticulously monitored during synthesis and controlled according to Bio-Synthesis’ stringent quality assurance and quality control standards. Each DNA template can be verified with bidirectional DNA sequencing in multiple locations. BSI’s Quality Control department will ensure the final product is verified and passes all standards.

For over 25 years, Bio‑Synthesis has maintained these gold standards for long RNA oligonucleotide synthesis that saves the researcher a great deal of time and trouble for applications that require direct synthesis of the entire target fragment such as mRNA.

Applications

These long oligos can be used in variety of molecular biology applications such as:

RNA control template for Real-Time PCR
Antisense technology
Internal controls for house-keeping genes
External RNA spike-in control assays
In-situ Hybridization RNA probes
Expression control via anti-sense RNA
RNA template for in vitro translation
RNA structural studies (Protein-RNA binding)
Ribozyme biochemistry
Aptamer discovery(SELEX)

Pricing

Purification

All Bio‑Synthesis RNA transcript oligos are purified under RNase free environment. Purification methods include:

Column based purification
Lithium chloride precipitation
Spin column chromatography
Phenol extraction followed by alcohol precipitation

Minimum Yield

Yield will vary based upon base composition, purification, oligo length, scale and modifications. That's why we have a set guaranteed quantity.

Guaranteed Quality

Each step of the RNA production process is carefully monitored, starting from oligonucleotide synthesis to DNA templates production. Each step is screened by mass spectrometry, agarose size check and bidirectional DNA sequencing in multiple locations. After transcription, the RNA is carefully checked using denaturing PAGE electrophoresis, Real-Time PCR and other additional custom assay developments as required by our customers.

If you are dissatisfied and report an oligo problem within 30 days of order receipt, we will gladly remake your oligo free of charge or provide a credit for the purchase amount.

All orders are shipped with a certificate of analysis and other fully traceable documentation.

RNA Long Oligonucleotide as Synthetic Pre-miRNA-Based shRNA

Bio‑Synthesis provides custom d2RNA^TM, in vitro transcribed long RNA (>3000 and more) and messenger RNA (mRNA) synthesis service and modifications with flexible synthesis scales for your individual research needs. The RNA probes produced at Bio‑Synthesis have been used as radio-labeled and nonisotopically labeled RNA probes in blot hybridization and nuclease protection assays. Modified RNA probes are used in various biochemical and molecular biology studies. d2RNA are also used as control template for gene expression analysis (microinjection, infection with viral transcripts, in vitro translation), structure analysis (protein-RNA binding), and RNA-based diagnostic testing and drug development.

d2RNA Service Highlights

Capped mRNAs or uncapped RNAs with different modifications
Multigrams quantity
Process scale-up and optimization
High yield in vitro transcripts from Plasmids, PCR Products, Oligonucleotides and cDNA
Multiple purification methods
Stringent quality control with optional custom assay development and validation
Delivered normalized and lyophilized
Ideal for gene functional analysis and the development of medical therapeutics

Left: Ambion’s Millennium Marker
Right: mecA RNA product

Technical Resources

Commonly Asked Questions:

Reference/Citing:

K Terasawa et al. " Synthetic Pre-miRNA-Based shRNA as Potent RNAi Triggers." Journal of Nucleic Acids, Volume 2011 (2011), article ID131479
B. Wang et al. "Synthetic long oligonucleotides to generate artificial templates for use as positive controls in molecular assays: drug resistance mutations in influenza virus as an example." Virology Journal 2011, 8:405
F. Yang et al. "Long non-coding RNA high expressed in hepatocellular carcinoma (lncRNA-HEIH) facilitates tumor growth through enhancer of zeste homolog 2. " Hepatology, 2011.
C. Braconi et al. " MicroRNA-29 can regulate expression of the long non-coding RNA gene MEG3 in hepatocellular cancer. " Oncogene. 2011, 1-7

Ordering & Contact Information

Please contact us for additional information or send an email to info@biosyn.com.
You may also request an online quote.
To contact us by phone, please Call 972-420-8505 (USA) 800-227-0627 (INTL.) or Fax 972.420.0442
Orders may be placed using a purchase order (PO) or by credit card through our secure online ordering system.
When using a credit card ( ) it will be billed under "Bio-Synthesis, Inc."

d2RNA - Oligo by Transcription

In Vitro Transcribed Long RNA