DeoxyUridine (dUracil) Modified Oligo

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Deoxy-uridine is incorporated in place of deoxyT under circumstances, the enzyme Uracil-N-glycosylase can specifically remove uracil to create baseless sites at the deoxyUridine positions. This property can be used to generate specific strand breaks in a DNA structure Click on specification page for details.

Desalt or cartridge (RP1) purification is acceptable for most common phosphoramidite modifiers. However, additional purification is strongly recommended for modifiers that require NHS-ester chemistry to conjugate a dye through an amine linker such as Molecular Probes dyes.

Due to the chemistry of many modifications, yield will be approximately 40 - 60 % less than a standard oligo. Please see our yield chart for details.

Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields are determined using UV absorbance at OD₂₆₀ In addition, we perform QC methods tailored to specific modifications, such as OD ratio measurement where appropriate.

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