Enhanced Diagnostic Tools
Deoxy-uridine is incorporated in place of deoxyT under circumstances, the enzyme
Uracil-N-glycosylase can specifically remove uracil to create baseless sites at
the deoxyUridine positions. This property can be used to generate specific strand
breaks in a DNA structure
Click on specification page for details.
Desalt or cartridge (RP1) purification is acceptable for most common phosphoramidite
modifiers. However, additional purification is strongly recommended for modifiers
that require NHS-ester chemistry to conjugate a dye through an amine linker such
as Molecular Probes dyes.
Due to the chemistry of many modifications, yield will be approximately 40 - 60
% less than a standard oligo. Please see our yield chart for details.
Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF
mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields
are determined using UV absorbance at OD260 In addition, we perform QC
methods tailored to specific modifications, such as OD ratio measurement where appropriate.
For additional information, please Contact us.