Enhanced Diagnostic Tools
Like biotin, digoxigenin (DIG) is mainly used as a non-isotopic label for DNA and
oligonucleotides in a wide range of applications. This small hapten can be conjugated
to amino-modified oligos with digoxigenin-NHS-ester. The positions of amino-modification
can be specified within an oligo. The 5' terminus can be labeled with a C6 or C12
linker. C12 may be preferable. Linker arm length determines accessibility of the
digoxigenin label to the detecting antibody. Amino-dT can be substituted for dT
at internal positions of the oligo. The spacing between labels should be maintained
at 10 or more bases to prevent steric limitations on antibody recognition of the
digoxigenin label. Multiple labels are necessary for northern and southern hybridization
Desalt or cartridge (RP1) purification is acceptable for most common phosphoramidite
modifiers. However, additional purification is strongly recommended for modifiers
that require NHS-ester chemistry to conjugate a dye through an amine linker such
as Molecular Probes dyes.
Due to the chemistry of many modifications, yield will be approximately 40 - 60
% less than a standard oligo. Please see our yield chart for details.
Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF
mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields
are determined using UV absorbance at OD260 In addition, we perform QC
methods tailored to specific modifications, such as OD ratio measurement where appropriate.
For additional information, please Contact us.