Why use peptide biotinylation?
Biotin (244 Da) can be added to your peptide at various positions. Due to the very strong non‐covalent interaction between biotin and avidin / streptavidin, it is useful to have this small molecule as a tag to immobilise the peptide, or to pull‐down the peptide from a mixture. Note that a spacer, such as a mini‐PEG or Ahx (aminohexanoic acid), between biotin and the N‐terminus give a better performance in biotechnological applications generally.