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Double DIG labels for higher sensitivity of in situ hybridization

Should I have double dig labels for microRNA in situ hybridization application?

Double (5’ and 3’) DIG-labeled probes offer substantially higher sensitivity than single labeled probes. A cooperative effect of the two DIG labels results in greatly increased signal to noise ratio (up to 10-fold higher) which means that even low abundance microRNAs can be reliably detected / We recommend this labeling option for optimal results for your microRNA in situ hybridization probes

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