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Can you label ATTO390 at internal oligo sequence?

Yes, Bio-Synthesis offers ATTO390 oligo labeling at 5' 3' and internally. ATTO390 can be labeled post-synthetically using NHS ester chemistry. NHS Esters react with free primary amines and result in stable, covalent attachment. A primary amine such as amino C6 modifier can be use to functionalize 5' and 3' oligo, amino dA, amino dT, amino dC, amino dG can be added internally to the oligo during synthesis to permit reaction with the desired NHS Ester. Post-synthetic chemical modifications made to an oligonucleotide result in lower yields than modifications introduced during synthesis. Further, all NHS Ester modification require dual HPLC purification.

Example of ATTO390 internal oligo labeling through NHS ester conjugation chemistry
ATTO 390 internal oligo modification