LNA bases can be used to make improved antisense chimeras in the same way that other 2´-modifications have been employed in the past, such as 2´-O-methyl RNA, MOE´s, etc. The use of LNAs offers improved Tm, increased resistance to cellular nucleases, and increased potency of the antisense oligo.
Kurreck, J., Wyszko, E., Gillen, C., and Erdmann, V.A. Design of antisense oligonucleotides stabilized by locked nucleic acids. Nucleic Acids Research, 30:1911-1918 (2002).
Braasch, D.A., Liu, Y., and Corey, D.R. Antisense inhibition of gene expression in cells by oligonucleotides incorporating locked nucleic acids: effect of mRNA target sequence and chimera design. Nucleic Acids Research, 30:5160-5167 (2002).