Dye selection guide for 488 nm laser

Alexa 488:

• Brighter and more photostable than FITC
• Interchangeable with FITC with only minor changes in compensation
• Excited at 488 nm with emission maximum at ~519 nm
• pH insensitive
• Ideal primary color for flow cytometry

• Excited at 488 nm or 532 nm with emission maximum at ~575 nm
• Very bright fluorescence
• Shows some excitation with 405 nm laser
• Highly susceptible to photobleaching

• Energy transfer dye excited at ~488 nm with emission maximum at ~615 nm
• Can be measured in FL3 channel on BD™ and Beckman Coulter instruments

• Energy transfer dye excited at ~488 nm with emission maximum at ~628 nm
• Alternative to RPE-Texas RedR dye
• Higher quantum yield than RPE-Texas RedR dye with lower compensation against RPE
• Ideal for use with RPE-Alexa FluorR 700 or RPE-CyR5.5 dyes in multicolor panels
• Can be measured in FL3 channel on BD™ and Beckman Coulter instruments
• Requires filter modification for optimal use on Beckman Coulter EPICSR XL™ instrument

• Fluorochrome of choice for BD™ FACSCalibur™ instrument
• Energy transfer dye excited at ~488 nm with emission maximum at ~723 nm

• Energy transfer dye excited at ~488 nm with emission maximum at ~694 nm
• Lower photostability than RPE-Alexa FluorR 700
• Requires more compensation against APC channel than RPE-Alexa FluorR 700
• Measured in FL3 channel on BD FACScan™ and BD FACSCalibur™ instruments
• Measured in FL4 on Beckman Coulter EPICSR XL™ instrument

• Provides long-wavelength emission off the 488 nm laser
• Energy transfer dye excited at ~488 nm with emission maximum at ~775 nm
• Requires varying degrees of compensation out of RPE channel