I would like to synthesize oligonucleotides of 60bp for antisense technology. I would like to be assured that you guarantee the sequence of the oligonucleotides synthesized.
We can assure sequence integrity by the following:
- Direct input of your sequence onto our instruments, eliminate operator transcription errors
- The oligos are purified by denaturing PAGE (which has single base resolution, at this point the oligos are>95% pure; the impurities would be the n-1 adducts, meaning that there still may be 5-10% of molecules with shorter/deletion sequences. We recommend double PAGE purification to drive this to >97% purity.
- As an option(probably not economically feasible for this short size; this is more advantageous when dealing with synthetic genes of >200 bp) we can sequence the 60 bp( because this is PCR sequence the last 1-2 bases of each strand will not show well, however these are the bases that we are the most certain about < since the chemical synthesis goes from 3’-5’>…all the other bases in between can be verified( using 2 primers in both directions)
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10/19/2009