Synthesize oligonucleotides of 60bp for antisense technology and assured the sequence of the oligonucleotides synthesized

We can assure sequence integrity by the following:

1. Direct input of your sequence onto our instruments, eliminate operator transcription errors
2. The oligos are purified by denaturing PAGE (which has single base resolution, at this point the oligos are>95% pure; the impurities would be the n-1 adducts, meaning that there still may be 5-10% of molecules with shorter/deletion sequences. We recommend double PAGE purification to drive this to >97% purity.
3. As an option(probably not economically feasible for this short size; this is more advantageous when dealing with synthetic genes of >200 bp) we can sequence the 60 bp( because this is PCR sequence the last 1-2 bases of each strand will not show well, however these are the bases that we are the most certain about < since the chemical synthesis goes from 3’-5’>…all the other bases in between can be verified( using 2 primers in both directions)