Explanation of peptide purity, peptide content and absolute amount of desired peptide.
Bio-Synthesis ships peptides according to the actual gross weight of the lyophilized product. This product includes any impurities such as fragments or deletion peptides, incompletely de-protected peptides, TFA, and residual water. Peptide purity measured by HPLC. It is the amount of correct peptide relative to all analytes that absorb at ~214 nm (the peptide bond absorbs), most likely deletion, truncation or incompletely deprotected sequences, etc. Peptide purity does not take into account water and salts that are usually present in the sample. Peptide content is the percentage of total peptides present in the product relative everything else present in the sample, such as water, salts, etc. Peptide content is determined by nitrogen content analysis. Absolute amount of desired peptide in the lyophilized peptide powder thus can be concluded by the formula: The peptide content X the peptide purity.
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05/08/2013