Enhanced Diagnostic Tools
In addition, most peptides are purified in the presence of trifluoroacetic acid (TFA) in the solvent and the amount of residual TFA and TFA salts in the peptide are difficult to quantify. Free TFA can generally be removed after lyophilization for at least 48 hours, but TFA salts with the peptide or with other buffer ions may be difficult to completely remove. If TFA or TFA salts are suspected to cause problems in the experiment, the customer should request specific buffers be used in the final purification and lyophilization steps.
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