Enhanced Diagnostic Tools
RNAi down regulates a gene function without actually interacting with the gene. The subtle action is by mRNA degradation. Thus the degree of RNA interference achieved is directly proportional to the level of mature mRNA and the translated proteins. The options are:
1. Measurements of target protein(enzyme) activity. This option is suitable if a robust assay is available or has been in prior use. The assay would vary by the nature of the protein product.
2. Measurement of target mRNA level. This is the preferred method as it directly quantifies the level of mRNA. Quantitative PCR is very effective in measuring relative amount of target sequence. This can be achieved simply by SYBR green or by the use of TaqMan or Molecular Beacons. More information.
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