Enhanced Diagnostic Tools
Lyophilized peptides may contain anywhere from 10% to as much as 70% bound water and salts by weight. More hydrophilic peptides generally contain more bound water and salts compared to hydrophobic peptides. Therefore, it is difficult to ascertain the actual peptide concentration based on the weight of the lyophilized peptide.
If the peptide has a chromophore in the sequence (W or Y residues), peptide concentration can be conveniently determined based on the extinction coefficient of these residues. The following steps can be used for the calculations:
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